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Titolo:
Synthesis and application of peptide immunogens related to the sperm tail protein tpx-1, a member of the CRISP superfamily of proteins
Autore:
Keah, HH; OBryan, MK; de Kretser, DM; Hearn, MTW;
Indirizzi:
Monash Univ, Ctr Bioproc Technol, Dept Biochem & Mol Biol, Clayton, Vic 3168, Australia Monash Univ Clayton Vic Australia 3168 Biol, Clayton, Vic 3168, Australia Monash Univ, Monash Med Ctr, Inst Reprod & Dev, Clayton, Vic 3168, Australia Monash Univ Clayton Vic Australia 3168 Dev, Clayton, Vic 3168, Australia
Titolo Testata:
JOURNAL OF PEPTIDE RESEARCH
fascicolo: 1, volume: 57, anno: 2001,
pagine: 1 - 10
SICI:
1397-002X(200101)57:1<1:SAAOPI>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
ANTIGENIC CROSS-REACTIVITY; THYROTROPIN BETA-SUBUNIT; PATHOGENESIS-RELATED PROTEINS; TESTIS-SPECIFIC GENE; MOLECULAR-CLONING; HUMAN CHORIOGONADOTROPIN; ANTIPEPTIDE ANTIBODIES; SEQUENCE SIMILARITY; HYMENOPTERA VENOM; DISULFIDE BONDS;
Keywords:
peptide immunogens; CRISP proteins; SPSS; ELISA; western immunoblot; rat tpx-1 proteins; ESI-MS; RP-HPLC;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Hearn, MTW Monash Univ, Ctr Bioproc Technol, Dept Biochem & Mol Biol, Clayton, Vic 3168, Australia Monash Univ Clayton Vic Australia 3168 on, Vic 3168, Australia
Citazione:
H.H. Keah et al., "Synthesis and application of peptide immunogens related to the sperm tail protein tpx-1, a member of the CRISP superfamily of proteins", J PEPT RES, 57(1), 2001, pp. 1-10

Abstract

The synthesis of peptides containing 0, 1 and 2 cysteine residues related to the human sperm tail protein, tpx-1, is described. These synthetic peptides, following conjugation to keyhole limpet hemocyanin modified with maleimidobenzoic acid N-hydroxysuccinimide ester, were used as immunogens to generate polyclonal antibodies in female New Zealand white rabbits. The binding characteristics of the derived antipeptide sera were evaluated using indirect and competitive ELISA procedures. Western immunoblot experiments also confirmed that these synthetic peptide immunogens are able to generate high-titer polyclonal antibodies capable of cross-reacting with the mature tpx-1 protein present in crude rat sperm tail/testis preparations as well as inouter dense fiber preparations. Consequently, these synthetic peptides represent promising candidates for investigations into the role of tpx-1 in the immunoregulation of sperm function in the rat and other mammalian models,with the derived antisera also providing an avenue to explore possible sites of expression of tpx-1 proteins in other tissues.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/20 alle ore 04:08:16