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Titolo:
Use of two anterograde axon tracers to label distinct cortical neuronal populations located in close proximity
Autore:
Ojima, H; Takayanagi, M;
Indirizzi:
RIKEN, BSI, Cort Org & Systemat, Wako, Saitama 3510198, Japan RIKEN Wako Saitama Japan 3510198 & Systemat, Wako, Saitama 3510198, Japan Toho Univ, Sch Med, Dept Anat 1, Ota Ku, Tokyo 1438940, Japan Toho Univ Tokyo Japan 1438940 Dept Anat 1, Ota Ku, Tokyo 1438940, Japan
Titolo Testata:
JOURNAL OF NEUROSCIENCE METHODS
fascicolo: 2, volume: 104, anno: 2001,
pagine: 177 - 182
SICI:
0165-0270(20010115)104:2<177:UOTAAT>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
PHASEOLUS-VULGARIS-LEUKOAGGLUTININ; VISUAL-CORTEX; DEXTRAN-AMINE; INTRINSIC CONNECTIONS; AUDITORY-CORTEX; IN-VIVO; CAT; INTEGRATION; BIOTIN; RAT;
Keywords:
biotinylated dextran amine; Phaseolus vulgaris leucoagglutinin; convergence; patchy plexus; auditory cortex; dual coloring; local network;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Ojima, H RIKEN, BSI, Cort Org & Systemat, 2-1 Hirosawa, Wako, Saitama 3510198, Japan RIKEN 2-1 Hirosawa Wako Saitama Japan 3510198 tama 3510198, Japan
Citazione:
H. Ojima e M. Takayanagi, "Use of two anterograde axon tracers to label distinct cortical neuronal populations located in close proximity", J NEUROSC M, 104(2), 2001, pp. 177-182

Abstract

In order to investigate: converging projections originating from adjacent populations of cortical neurons, injections of two different anterograde tracers, biotinylated dextranamine (BDA) and Phaseolus vulgaris leucoagglutinin (PHA-L), were made in close proximity. When the two injection sites wereseparated by around 500 mum and the time between injections was 1-4 h, BDA-labeling of neuronal elements was found not only at the BDA injection sitebut also at the PHA-L injection site. This false-positive BDA labeling of neurons at the PI-IA-L injection site was so intense that labeled axons could be traced, both into the neighboring cortical gray matter and into whitematter. Increasing the separation distance to 1000 mum resulted in much fewer falsely positive labeled neurons at the PHA-L injection site. Even moreeffective was: extending the time interval between the two injections. Thus, if the BDA injection preceded the PHA-L injection by more than 12 h, virtually no false-positive labeling was associated with the PHA-L injection site. Those procedures may be applied to other combinations of anterograde tracers, such as BDA with tetramethylrhodamine-conjugated dextran amine. (C)2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/09/20 alle ore 03:19:24