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Titolo:
Mode and tempo of tangential cell migration in the cerebellar external granular layer
Autore:
Komuro, H; Yacubova, E; Yacubova, E; Rakic, P;
Indirizzi:
Cleveland Clin Fdn, Lerner Res Inst, Dept Neurosci NC30, Cleveland, OH 44195 USA Cleveland Clin Fdn Cleveland OH USA 44195 i NC30, Cleveland, OH 44195 USA Yale Univ, Sch Med, Neurobiol Sect, New Haven, CT 06510 USA Yale Univ NewHaven CT USA 06510 Neurobiol Sect, New Haven, CT 06510 USA
Titolo Testata:
JOURNAL OF NEUROSCIENCE
fascicolo: 2, volume: 21, anno: 2001,
pagine: 527 - 540
SICI:
0270-6474(20010115)21:2<527:MATOTC>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
INTRACELLULAR CA2+ FLUCTUATIONS; MULTIPLE ADHESION MOLECULES; DEVELOPING CHICK CEREBELLUM; GUIDED NEURONAL MIGRATION; PURKINJE-CELLS; CORTEX; DIFFERENTIATION; SPECIFICATION; RECEPTORS; PATTERNS;
Keywords:
cerebellar development; granule cell; neuronal cell migration; confocal microscopy; brain slice preparation; fluorescent carbocyanine dye; rate of cell movement;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
57
Recensione:
Indirizzi per estratti:
Indirizzo: Komuro, H Cleveland Clin Fdn, Lerner Res Inst, Dept Neurosci NC30, 9500 Euclid Ave, Cleveland, OH 44195 USA Cleveland Clin Fdn 9500 Euclid Ave Cleveland OH USA 44195 95 USA
Citazione:
H. Komuro et al., "Mode and tempo of tangential cell migration in the cerebellar external granular layer", J NEUROSC, 21(2), 2001, pp. 527-540

Abstract

After their final mitosis, cerebellar granule cells remain in the externalgranular layer (EGL) for 20-48 hr before initiating their radial migrationacross the molecular layer (ML), but the significance of this latent period is not well understood. In the present study, we used a confocal microscope to examine morphogenetic changes and behavior of postmitotic granule cells restricted to the EGL in slice preparations of the postnatal mouse cerebellum. We found that, coincident with the extension of two uneven horizontal processes oriented parallel to the longitudinal axis of the folium, postmitotic granule cells start to migrate tangentially in the direction of the larger process. Interestingly, their morphology and the speed of cell movement change systematically with their position within the EGL. The rate of tangential cell movement is fastest (similar to 14.8 mum/hr) in the middle of the EGL, when cells have two short horizontal processes. As granule cellselongate their somata and extend longer horizontal processes at the bottomof the EGL, they move at a reduced rate (similar to 12.6 mum/hr). At the interface of the EGL and ML where cells migrate tangentially at the slowest rate (similar to4.1 mum/hr), their somata round and then begin to extend couples of the descending processes into the ML. After the stationary period,granule cells abruptly extend a single vertical process and initiate the transition from tangential to radial migration, reshaping their rounded somata into a vertically elongated spindle. These observations suggest that tangential migration of granule cells within the EGL may provide the developmental mechanisms for their appropriate allocation across parasagittal compartments of the expanding cerebellar cortex.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 23:42:16