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Titolo:
THE EFFECT OF VIRAL REGULATORY PROTEIN EXPRESSION ON GENE DELIVERY BYHUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 VECTORS PRODUCED IN STABLE PACKAGING CELL-LINES
Autore:
SRINIVASAKUMAR N; CHAZAL N; HELGAMARIA C; PRASAD S; HAMMARSKJOLD ML; REKOSH D;
Indirizzi:
UNIV VIRGINIA,MYLES H THALER CTR AIDS & HUMAN RETROVIRUS RES CHARLOTTESVILLE VA 22908 UNIV VIRGINIA,MYLES H THALER CTR AIDS & HUMAN RETROVIRUS RES CHARLOTTESVILLE VA 22908 UNIV VIRGINIA,DEPT MICROBIOL CHARLOTTESVILLE VA 22908
Titolo Testata:
Journal of virology
fascicolo: 8, volume: 71, anno: 1997,
pagine: 5841 - 5848
SICI:
0022-538X(1997)71:8<5841:TEOVRP>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
LATE REPLACEMENT VECTOR; EPSTEIN-BARR VIRUS; CIS-ACTING ELEMENT; MESSENGER-RNA; REV PROTEIN; SYNCYTIUM FORMATION; RETROVIRAL VECTORS; HIV-1 GAG; HTLV-III; REPLICATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
61
Recensione:
Indirizzi per estratti:
Citazione:
N. Srinivasakumar et al., "THE EFFECT OF VIRAL REGULATORY PROTEIN EXPRESSION ON GENE DELIVERY BYHUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 VECTORS PRODUCED IN STABLE PACKAGING CELL-LINES", Journal of virology, 71(8), 1997, pp. 5841-5848

Abstract

We describe the generation of stable human immunodeficiency virus type 1 (HIV-1)-packaging lines that constitutively express high levels ofHIV-1 structural proteins in either a Rev-dependent or a Rev-independent fashion. These cell lines were used to assess gene transfer by using an HIV-1 vector expressing the hygromycin B resistance gene and to study the effects of Rev, Tat, and Nef on the vector titer. The Rev-independent cell lines were created by using gag-pol and env expression vectors that contain the Mason-Pfizer monkey virus (MPMV) constitutivetransport element (CTE). Vector titers approaching 10(4) CFU/ml mere routinely obtained with these cell lines, as well as with the Rev-dependent cell lines, with HeLa-CD4 cells as targets. The presence of Nef and Tat in the producer cell each increased the vector titer 5- to 10-fold. Rev, on the other hand, was absolutely essential for gene transfer, unless the MPMV CTE was present in the vector. In that case, by using the Rev-independent cell lines for packaging, Rev could be completely eliminated from the system without a reduction in vector titer.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 07:29:05