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Titolo:
Immunolocalization of cytoplasmic dynein and dynactin subunits in culturedmacrophages: enrichment on early endocytic organelles
Autore:
Habermann, A; Schroer, TA; Griffiths, G; Burkhardt, JK;
Indirizzi:
Univ Chicago, Dept Pathol, Chicago, IL 60637 USA Univ Chicago Chicago IL USA 60637 ago, Dept Pathol, Chicago, IL 60637 USA European Mol Biol Lab, Cell Biol Programme, D-69012 Heidelberg, Germany European Mol Biol Lab Heidelberg Germany D-69012 012 Heidelberg, Germany Johns Hopkins Univ, Dept Biol, Baltimore, MD 21218 USA Johns Hopkins UnivBaltimore MD USA 21218 t Biol, Baltimore, MD 21218 USA
Titolo Testata:
JOURNAL OF CELL SCIENCE
fascicolo: 1, volume: 114, anno: 2001,
pagine: 229 - 240
SICI:
0021-9533(200101)114:1<229:IOCDAD>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACTIN-RELATED PROTEIN; SCATTERED GOLGI ELEMENTS; CELL-CYCLE REGULATION; MOLECULAR CHARACTERIZATION; AXONAL-TRANSPORT; MOTOR PROTEINS; MICROTUBULE DISRUPTION; NUCLEAR-DISTRIBUTION; SPINDLE FORMATION; EPITHELIAL-CELLS;
Keywords:
electron microscopy; microtubule; motor protein; organelle motility;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
84
Recensione:
Indirizzi per estratti:
Indirizzo: Burkhardt, JK Univ Chicago, Dept Pathol, 5841 S Maryland Ave, Chicago, IL 60637 USA Univ Chicago 5841 S Maryland Ave Chicago IL USA 60637 37 USA
Citazione:
A. Habermann et al., "Immunolocalization of cytoplasmic dynein and dynactin subunits in culturedmacrophages: enrichment on early endocytic organelles", J CELL SCI, 114(1), 2001, pp. 229-240

Abstract

Cytoplasmic dyneins and their cofactor, dynactin, work together to mediatethe movement of numerous cargo organelles toward the minus-ends of microtubules. In many cases, there is compelling evidence that dynactin functions in part to attach dyneins to cargo organelles, but this may not always be the case, We have localized three dynactin subunits (Arp1, p62 and p150(Glued)) and two subunits of conventional cytoplasmic dynein (dynein intermediate chain and dynein heavy chain 1) in murine macrophages using immunogold labeling of thawed cryosections. Using stereological techniques, we have quantified the relative distributions of each of these subunits on specific membrane organelles to generate a comprehensive analysis of the distribution of these proteins in a single cell type. Our results show that each of the subunits tested exhibits the same distribution with respect to different membrane organelles, with highest levels present on early endosomes, and lowerlevels present on later endocytic organelles, the mitochondrial outer membrane, the plasma membrane and vesicles in the Golgi region, An additional pool of punctate dynactin labeling was detected in the cell periphery, in the absence of dynein labeling, Even when examined closely, membrane organelles could not be detected in association with these dynactin-positive sites;however, double labeling with anti-tubulin antibody revealed that at leastsome of these sites represent the ends of microtubules, The similarities among the labeling profiles with respect to membrane organelles suggest thatdynein and dynactin bind to membrane organelles as an obligate unit, In contrast, our results show that dynactin can associate with microtubule ends in the absence of dynein, perhaps providing sites for subsequent organelle and dynein association to form a functional motility complex.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 13:01:44