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Titolo:
Alkaline response genes of Saccharomyces cerevisiae and their relationshipto the RIM101 pathway
Autore:
Lamb, TM; Xu, WJ; Diamond, A; Mitchell, AP;
Indirizzi:
Columbia Univ, Dept Microbiol, New York, NY 10032 USA Columbia Univ New York NY USA 10032 ept Microbiol, New York, NY 10032 USA Columbia Univ, Canc Res Inst, New York, NY 10032 USA Columbia Univ New York NY USA 10032 Canc Res Inst, New York, NY 10032 USA Columbia Univ, Integrated Program Cellular Mol & Biophys Studies, New York, NY 10032 USA Columbia Univ New York NY USA 10032 ophys Studies, New York, NY 10032 USA
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 3, volume: 276, anno: 2001,
pagine: 1850 - 1856
SICI:
0021-9258(20010119)276:3<1850:ARGOSC>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
FINGER TRANSCRIPTION FACTOR; MEMBRANE H+-ATPASE; PH-REGULATED GENE; CANDIDA-ALBICANS; PLASMA-MEMBRANE; AMBIENT PH; PHOSPHATASE CALCINEURIN; BINDING-SITES; ENA1 GENE; YEAST;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
59
Recensione:
Indirizzi per estratti:
Indirizzo: Mitchell, AP Columbia Univ, Dept Microbiol, 701 W 168th St, New York, NY 10032 USA Columbia Univ 701 W 168th St New York NY USA 10032 10032 USA
Citazione:
T.M. Lamb et al., "Alkaline response genes of Saccharomyces cerevisiae and their relationshipto the RIM101 pathway", J BIOL CHEM, 276(3), 2001, pp. 1850-1856

Abstract

Environmental pH exerts broad control over growth and differentiation, butthe molecular responses to external pH changes are poorly understood. Herewe have used open reading frame macroarray hybridization to identify alkaline response genes in Saccharomyces cer evisiae. Northern or lacZ fusion assays confirmed the alkaline induction of two ion pump genes (ENA1 and VMA4), several ion limitation genes (CTR3, FRE1, PHO11/12, and PHO84), a siderophore-iron transporter gene (ARN4/ENB1), two transcription factor genes (NRG2 and TIS11), and two predicted membrane protein genes (YAR068W/YHR214W andYOL154W). Unlike ENA1 and SHC1, these new alkaline response genes are not induced by high salinity. The known pH-responsive genes in other fungi depend on the conserved PacC/Rim101p transcription factor, but induction of several of these new genes relied upon Rim101p-independent pH signaling mechanisms. Rim101p-dependent genes were also dependent on Rim13p, a protease required for Rim101p processing. The Rim101p-dependent gene VMA4 is required for growth in alkaline conditions, illustrating how Rim101p may control adaptation. Because Rim101p activates ion pump genes, we tested the role of RIM101 in ion homeostasis and found that RIM101 promotes resistance to elevated cation concentrations. Thus, gene expression surveys can reveal new functions for characterized transcription factors in addition to uncovering physiological responses to environmental conditions.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 12/08/20 alle ore 20:49:28