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Titolo:
Early detection of reverse transcriptase activity in plasma of neonates infected with HIV-1: A comparative analysis with RNA-based and DNA-based testing using polymerase chain reaction
Autore:
Reisler, RB; Thea, DM; Pliner, V; Green, T; Lee, F; Nesheim, S; Brown, T; Kalish, M; Folks, TM; Heneine, W;
Indirizzi:
Ctr Dis Control & Prevent, HIV & Retrovirol Branch, Div AIDS STD & TB Lab Res, Atlanta, GA 30333 USA Ctr Dis Control & Prevent Atlanta GA USA 30333 Res, Atlanta, GA 30333 USA Emory Univ, Dept Med, Div Infect Dis, Atlanta, GA 30322 USA Emory Univ Atlanta GA USA 30322 ed, Div Infect Dis, Atlanta, GA 30322 USA Med & Hlth Res Associat, New York, NY USA Med & Hlth Res Associat New York NY USA h Res Associat, New York, NY USA CDC, Off Director, DASTLR, Atlanta, GA 30333 USA CDC Atlanta GA USA 30333CDC, Off Director, DASTLR, Atlanta, GA 30333 USA Emory Univ, Dept Pediat, Atlanta, GA 30322 USA Emory Univ Atlanta GA USA 30322 Univ, Dept Pediat, Atlanta, GA 30322 USA
Titolo Testata:
JOURNAL OF ACQUIRED IMMUNE DEFICIENCY SYNDROMES
fascicolo: 1, volume: 26, anno: 2001,
pagine: 93 - 102
SICI:
1525-4135(20010101)26:1<93:EDORTA>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
IMMUNODEFICIENCY-VIRUS TYPE-1; DISEASE PROGRESSION; TRUNCATED DATA; RISK-FACTORS; P24 ANTIGEN; VIRAL LOAD; INFANTS; TRANSMISSION; CHILDREN; DIAGNOSIS;
Keywords:
viral load; HIV-1 DNA; PCR-HIV-1; RNA PCR; Amp-RT reverse transcriptase; neonates; PACTS; HIV-1 diagnosis; HIV-1 prognosis; sensitivity;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
44
Recensione:
Indirizzi per estratti:
Indirizzo: Heneine, W Ctr Dis Control & Prevent, HIV & Retrovirol Branch, Div AIDS STD & TB Lab Res, 1600 Clifton Rd NE,MS G-19, Atlanta, GA 30333 USA Ctr Dis Control & Prevent 1600 Clifton Rd NE,MS G-19 Atlanta GA USA 30333
Citazione:
R.B. Reisler et al., "Early detection of reverse transcriptase activity in plasma of neonates infected with HIV-1: A comparative analysis with RNA-based and DNA-based testing using polymerase chain reaction", J ACQ IMM D, 26(1), 2001, pp. 93-102

Abstract

Plasma viral load from 71 HIV-1-infected neonates was measured by using Amp-RT, an ultrasensitive quantitative reverse transcriptase (RT) assay and by nucleic acid sequence-based amplification (NASBA), an RNA-based quantitative assay. Results were then compared with those obtained from detection ofproviral DNA in peripheral blood mononuclear cells (PBMCs) by polymerase chain reaction (PCR) using Turnbull analysis. At 5 days of life, 50% of neonates were positive by Amp-RT, 30% were NASBA positive, and 20% were DNA-PCRpositive. Through the first 12 days of life, Amp-RT was more sensitive than either NASBA or DNA-PCR in detecting HIV-1 infection. Amp-RT values correlated well with NASBA RNA values, with an overall Pearson's r = 0.63 (95% confidence interval [CI], 0.40-0.78). In proportional hazards analysis of infants aged 14 to 61 days (N = 31), a one-log increase in RNA-based viral load was associated with a > fivefold risk of disease progression when using the U.S. Centers for Disease Control and Prevention (CDC) clinical CategoryC (CDC-C) or death as an endpoint (p = .014). Kaplan-Meier analysis of these data found that RNA viral loads were able to predict disease progressionusing CDC-C/death as an endpoint (p = .013). Early quantitative viral loadmeasurements may assist clinicians in diagnosing HIV-1 infection, stratifying risk of disease progression, and implementing a treatment plan using highly active antiretroviral therapy for infants within the first few weeks of life.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/20 alle ore 12:12:48