Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Identification of sequences in the human peptide transporter subunit TAP1 required for transporter associated with antigen processing (TAP) function
Autore:
Ritz, U; Momburg, F; Pircher, HP; Strand, D; Huber, C; Seliger, B;
Indirizzi:
Univ Mainz, Dept Internal Med 3, D-55131 Mainz, Germany Univ Mainz MainzGermany D-55131 Internal Med 3, D-55131 Mainz, Germany German Canc Res Ctr, D-69120 Heidelberg, Germany German Canc Res Ctr Heidelberg Germany D-69120 69120 Heidelberg, Germany Univ Freiburg, Dept Immunol, D-79104 Freiburg, Germany Univ Freiburg Freiburg Germany D-79104 mmunol, D-79104 Freiburg, Germany Univ Mainz, Dept Internal Med 1, D-55131 Mainz, Germany Univ Mainz MainzGermany D-55131 Internal Med 1, D-55131 Mainz, Germany
Titolo Testata:
INTERNATIONAL IMMUNOLOGY
fascicolo: 1, volume: 13, anno: 2001,
pagine: 31 - 41
SICI:
0953-8178(200101)13:1<31:IOSITH>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
CLASS-I MOLECULES; RMA-S CELLS; NUCLEOTIDE-BINDING; MEMBRANE TOPOLOGY; VIRAL-ANTIGEN; REGION; GENE; INHIBITOR; MUTATION; PROTEIN;
Keywords:
antigen presentation; antigen processing; MHC; peptide transporter; T cell response;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Seliger, B Univ Mainz, Dept Internal Med 3, Langenbeckstr 1, D-55131 Mainz, Germany Univ Mainz Langenbeckstr 1 Mainz Germany D-55131 ainz, Germany
Citazione:
U. Ritz et al., "Identification of sequences in the human peptide transporter subunit TAP1 required for transporter associated with antigen processing (TAP) function", INT IMMUNOL, 13(1), 2001, pp. 31-41

Abstract

The heterodimeric peptide transporter associated with antigen processing (TAP) consisting of the subunits TAP1 and TAP2 mediates the transport of cytosolic peptides into the lumen of the endoplasmic reticulum (ER), in order to accurately define domains required for peptide transporter function, a molecular approach based on the construction of a panel of human TAP1 mutants and their expression in TAP1(-/-) cells was employed, The characteristicsand biological activity of the various TAP1 mutants were determined, and compared to that of wild-type TAP1 and TAP1(-/-) control cells, All mutant TAP1 proteins were localized in the ER and were capable of forming complexeswith the TAPP subunit, However, the TAP1 mutants analyzed transported peptides with different efficiencies and displayed a heterogeneous MHC class I surface expression pattern which was directly associated with their susceptibility to cytotoxic T lymphocyte-mediated lysis, Based on this study, the TAP1 mutants can be divided into three categories: those expressing a similar phenotype compared to TAP1(-/-) or wild-type TAP1 cells respectively, and those representing an intermediate phenotype in terms of peptide transport rate, MHC class I surface expression and immune recognition. Thus, the results provide evidence that specific regions in the TAP1 subunit are crucial for the proper processing and presentation of cytosolic antigens to MHC class I-restricted T cells, whereas others may play a minor role in this process.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/06/20 alle ore 09:29:32