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Titolo:
Analysis of C-MYC function in normal cells via conditional gene-targeted mutation
Autore:
de Alboran, IM; OHagan, RC; Gartner, F; Malynn, B; Davidson, L; Rickert, R; Rajewsky, K; DePinho, RA; Alt, FW;
Indirizzi:
Harvard Univ, Sch Med, Howard Hughes Med Inst, Boston, MA 02115 USA Harvard Univ Boston MA USA 02115 rd Hughes Med Inst, Boston, MA 02115 USA Harvard Univ, Sch Med, Childrens Hosp, Ctr Blood Res, Boston, MA 02115 USAHarvard Univ Boston MA USA 02115 osp, Ctr Blood Res, Boston, MA 02115 USA Harvard Univ, Sch Med, Dept Genet, Boston, MA 02115 USA Harvard Univ Boston MA USA 02115 ch Med, Dept Genet, Boston, MA 02115 USA Univ Calif San Diego, Dept Biol, La Jolla, CA 92093 USA Univ Calif San Diego La Jolla CA USA 92093 t Biol, La Jolla, CA 92093 USA Univ Cologne, Inst Genet, D-50931 Cologne, Germany Univ Cologne Cologne Germany D-50931 nst Genet, D-50931 Cologne, Germany Harvard Univ, Sch Med, Dana Farber Canc Inst, Dept Adult Oncol, Boston, MA02115 USA Harvard Univ Boston MA USA 02115 t, Dept Adult Oncol, Boston, MA02115 USA Harvard Univ, Sch Med, Dept Med & Genet, Boston, MA 02115 USA Harvard Univ Boston MA USA 02115 , Dept Med & Genet, Boston, MA 02115 USA
Titolo Testata:
IMMUNITY
fascicolo: 1, volume: 14, anno: 2001,
pagine: 45 - 55
SICI:
1074-7613(200101)14:1<45:AOCFIN>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
EXPRESSION INDUCES APOPTOSIS; B-CELLS; TRANSGENIC MICE; GROWTH ARREST; CYCLE PROGRESSION; PROTEIN-SYNTHESIS; ANTIGEN RECEPTOR; T-CELLS; IN-VIVO; FIBROBLASTS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
60
Recensione:
Indirizzi per estratti:
Indirizzo: Alt, FW Harvard Univ, Sch Med, Howard Hughes Med Inst, Boston, MA 02115 USA Harvard Univ Boston MA USA 02115 s Med Inst, Boston, MA 02115 USA
Citazione:
I.M. de Alboran et al., "Analysis of C-MYC function in normal cells via conditional gene-targeted mutation", IMMUNITY, 14(1), 2001, pp. 45-55

Abstract

Germline inactivation of c-myc in mice causes embryonic lethality. Therefore, we developed a LoxP/Cre-based conditional mutation approach to test therole of c-myc in mouse embryonic fibroblasts (MEFs) and mature B lymphocytes. Cre expression resulted in reduced proliferation of wild-type MEFs, butc-Myc-deficient MEFs showed a further reduction. In contrast to fibroblasts, Cre expression had no apparent affect on wild-type B cell proliferation. Deletion of both c-Myc genes in B cells led to severely impaired proliferation in response to anti-CD40 plus IL-4. However, treated cells did upregulate several early activation markers but not CD95 or CD95 ligand. We discuss these findings with respect to potential c-Myc functions in proliferationand apoptosis and also discuss potential limitations in the Cre-mediated gene inactivation approach.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 13/07/20 alle ore 17:55:56