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Titolo:
Effect of renin-angiotensin system blockade on the expression of the angiotensinogen gene and induction of hypertrophy in rat kidney proximal tubularcells
Autore:
Zhang, SL; To, C; Chen, X; Filep, JG; Tang, SS; Ingelfinger, JR; Carriere, S; Chen, JSD;
Indirizzi:
Univ Montreal, Maisonneuve Rosemont Hosp, Res Ctr, Montreal, PQ H1T 2M4, Canada Univ Montreal Montreal PQ Canada H1T 2M4 tr, Montreal, PQ H1T 2M4, Canada Harvard Univ, Sch Med, Massachusetts Gen Hosp, Pediat Nephrol Unit, Boston, MA USA Harvard Univ Boston MA USA Gen Hosp, Pediat Nephrol Unit, Boston, MA USA Servier Amer, Laval, PQ, Canada Servier Amer Laval PQ CanadaServier Amer, Laval, PQ, Canada
Titolo Testata:
EXPERIMENTAL NEPHROLOGY
fascicolo: 2, volume: 9, anno: 2001,
pagine: 109 - 117
SICI:
1018-7782(200103/04)9:2<109:EORSBO>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
CELLULAR HYPERTROPHY; MESSENGER-RNA; DIABETIC NEPHROPATHY; BETA; TRANSCRIPTION; ANTAGONISTS; SECRETION; RECEPTOR; FEEDBACK; SEQUENCE;
Keywords:
hyperglycemia; angiotensinogen; kidney and hypertrophy;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Chen, JSD Univ Montreal, Maisonneuve Rosemont Hosp, Res Ctr, 5415 Boul Assompt, Montreal, PQ H1T 2M4, Canada Univ Montreal 5415 Boul Assompt MontrealPQ Canada H1T 2M4 anada
Citazione:
S.L. Zhang et al., "Effect of renin-angiotensin system blockade on the expression of the angiotensinogen gene and induction of hypertrophy in rat kidney proximal tubularcells", EXP NEPHROL, 9(2), 2001, pp. 109-117

Abstract

Studies have shown that high levels of glucose and angiotensin II (Ang II)stimulate hypertrophy and the expression of matrix protein genes in mouse proximal tubular cells in vitro. The present study tested the hypothesis that blockade of the renin-angiotensin system (RAS) inhibits the stimulatory effect of high levels of glucose on the expression of the renal angiotensinogen (ANG) gene and the formation of Ang II and subsequently attenuates theinduction of hypertrophy in kidney proximal tubular cells. Immortalized rat proximal tubular cells (IRPTC) were cultured in monolayer. The levels of expression of rat ANG and ANG mRNA in the IRPTC were quantified by specificradioimmunoassays for rat ANG (RIA-rANG) and by a reverse-transcription polymerase chain reaction (RT-PCR) assay, respectively. Hypertrophy of IRPTC was analyzed by flow cytometry (FACScan) and cellular protein assay. Our studies showed that losartan (an Ang II (AT(1))-receptor blocker), perindopril and captopril (inhibitors of angiotensin-converting enzyme) blocked the stimulatory effect of a high level of glucose (i.e. 25 mM) on the expressionof the rat ANG gene and hypertrophy in IRPTC but not by the Ang II (AT(2))-receptor blocker. Our studies indicate that the blockade of RAS is effective in inhibiting the stimulatory effect of hyperglycemia on the expression of the ANG gene and hypertrophy in IRPTC, supporting the notion that the local formation of intrarenal Ang II may play a role in the development of renal hypertrophy during early diabetes. Copyright (C) 2001 S. Karger AG, Basel.

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Documento generato il 02/04/20 alle ore 12:36:04