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Titolo:
Identification of NKp80, a novel triggering molecule expressed by human NKcells
Autore:
Vitale, M; Falco, M; Castriconi, R; Parolini, S; Zambello, R; Semenzato, G; Biassoni, R; Bottino, C; Moretta, L; Moretta, A;
Indirizzi:
Univ Genoa, Dipartimento Med Sperimentale, Sez Istol, I-16132 Genoa, ItalyUniv Genoa Genoa Italy I-16132 imentale, Sez Istol, I-16132 Genoa, Italy Ist Nazl Ric Canc, I-16132 Genoa, Italy Ist Nazl Ric Canc Genoa Italy I-16132 azl Ric Canc, I-16132 Genoa, Italy Univ Brescia, Dipartimento Sci Biomed & Biotechnol, Brescia, Italy Univ Brescia Brescia Italy ento Sci Biomed & Biotechnol, Brescia, Italy Univ Padua, Dipartimento Med Clin & Sperimentale, Padua, Italy Univ PaduaPadua Italy partimento Med Clin & Sperimentale, Padua, Italy Ist Giannina Gaslini, I-16148 Genoa, Italy Ist Giannina Gaslini Genoa Italy I-16148 a Gaslini, I-16148 Genoa, Italy
Titolo Testata:
EUROPEAN JOURNAL OF IMMUNOLOGY
fascicolo: 1, volume: 31, anno: 2001,
pagine: 233 - 242
SICI:
0014-2980(200101)31:1<233:IONANT>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
NATURAL-KILLER-CELLS; CLASS-I MOLECULES; LYMPHOPROLIFERATIVE DISEASE; GRANULAR LYMPHOCYTES; IMMUNOGLOBULIN SUPERFAMILY; INHIBITORY RECEPTOR; SURFACE-MOLECULE; T-CELLS; HLA-E; ACTIVATION;
Keywords:
NK cell; activating receptor; cell-mediated cytotoxicity; C-type lectin molecule; lymphoproliferative disease of granular lymphocytes;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Moretta, A Univ Genoa, Dipartimento Med Sperimentale, Sez Istol, Via GB Marsano 10, I-16132 Genoa, Italy Univ Genoa Via GB Marsano 10 Genoa Italy I-16132 Genoa, Italy
Citazione:
M. Vitale et al., "Identification of NKp80, a novel triggering molecule expressed by human NKcells", EUR J IMMUN, 31(1), 2001, pp. 233-242

Abstract

The ability of NK cells to kill a wide range of tumor or virally infected target cells as well as normal allogeneic T cell blasts appears to depend upon the concerted action of multiple triggering NK receptors. In this study, using two specific monoclonal antibodies [(mAb) MA152 and LAP171], we identified a triggering NK receptor expressed at the cell surface as a dimer of approximately 80 kDa (NKp80). NKp80 is expressed by virtually all fresh or activated NK cells and by a minor subset of T cells characterized by the CD56 surface antigen. NKp80 surface expression was also detected in all CD3(-) and in 6/10 CD3(+) large granular lymphocyte expansions derived from patients with lymphoproliferative disease of granular lymphocytes. In polyclonal NK cells, mAb-mediated cross-linking of NKp80 resulted in induction of cytolytic activity and Ca2+ mobilization. A marked heterogeneity existed inthe magnitude of the cytolytic responses of different NK cell clones to anti-NKp80 mAb. This heterogeneity correlated with the surface density of NKp46 molecules expressed by different NK clones. The mAb-mediated masking of NKp80 led to a partial inhibition of the NK-mediated lysis of appropriate allogeneic phytohemagglutinin-induced T cell blasts, while it had no effect on the lysis of different tumor target cells, including T cell leukemia cells. These data suggest that NKp80 recognizes a ligand on normal T cells that may be down-regulated during tumor transformation. Molecular cloning of the cDNA coding for NKp80 revealed a type II transmembrane molecule of 231 amino acids identical to the putative protein encoded by a recently identified cDNA termed KLRF1.

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Documento generato il 29/10/20 alle ore 20:46:07