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Titolo:
Cloning of three new allergens from the dust mite Lepidoglyphus destructorusing phage surface display technology
Autore:
Eriksson, TLJ; Rasool, O; Huecas, S; Whitley, P; Crameri, R; Appenzeller, U; Gafvelin, G; van Hage-Hamsten, M;
Indirizzi:
Karolinska Hosp, Allergy & Clin Immunol Unit, S-17176 Stockholm, Sweden Karolinska Hosp Stockholm Sweden S-17176 Unit, S-17176 Stockholm, Sweden Karolinska Inst, Allergy & Clin Immunol Unit, Stockholm, Sweden KarolinskaInst Stockholm Sweden & Clin Immunol Unit, Stockholm, Sweden Univ Madrid, Dept Biochem & Mol Biol, Madrid, Spain Univ Madrid Madrid Spain Madrid, Dept Biochem & Mol Biol, Madrid, Spain Univ Bath, Dept Biol & Biochem, Bath BA2 7AY, Avon, England Univ Bath Bath Avon England BA2 7AY Biochem, Bath BA2 7AY, Avon, England Swiss Inst Allergy & Asthma Res, CH-7270 Davos, Switzerland Swiss Inst Allergy & Asthma Res Davos Switzerland CH-7270 s, Switzerland
Titolo Testata:
EUROPEAN JOURNAL OF BIOCHEMISTRY
fascicolo: 2, volume: 268, anno: 2001,
pagine: 287 - 294
SICI:
0014-2956(200101)268:2<287:COTNAF>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
ANTIBODY-BINDING SPECIFICITIES; STORAGE MITES; HOUSE-DUST; ACARUS-SIRO; DERMATOPHAGOIDES-PTERONYSSINUS; TYROPHAGUS-PUTRESCENTIAE; BLOMIA-TROPICALIS; MAJOR ALLERGEN; SENSITIZATION; IGE;
Keywords:
Ld 5; Lep d 13; Lep d 7; Lepidoglyphus destructor; phage display;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Eriksson, TLJ Karolinska Hosp, Allergy & Clin Immunol Unit, S-17176 Stockholm, Sweden Karolinska Hosp Stockholm Sweden S-17176 Stockholm, Sweden
Citazione:
T.L.J. Eriksson et al., "Cloning of three new allergens from the dust mite Lepidoglyphus destructorusing phage surface display technology", EUR J BIOCH, 268(2), 2001, pp. 287-294

Abstract

The dust mite Lepidoglyphus destructor is a common species in Europe and amajor cause of dust mite allergy in rural surroundings, but it also contributes to dust mite allergy in urban areas. One major allergen, Lep d 2, hasbeen expressed as a recombinant protein and evaluated both in vivo and in vitro and shown to detect 60% or more of L. destructor-sensitized subjects. Additional recombinant allergens are needed to obtain a reliable diagnostic tool for L. destructor allergy. The aim of this study was to clone and express new allergens from L. destructor and determine their recognition frequency among sensitized individuals. A phage display cDNA expression librarywas constructed and screened with sera from L. destructor-sensitized individuals. The cDNAs encoding the allergens were cloned into the pET17b vectorand subsequently expressed in Escherichia coli as C-terminal His(6)-taggedproteins. Immunoblotting of the recombinant proteins was performed using sera from 45 subjects allergic to L. destructor. Three new allergens from L.destructor, Ld 5 (originating from a partial Lep d 5 clone), Lep d 7 and Lep d 13, were identified and recognized by 4/45 (9%), 28/45 (62%) and 6/45 (13%) sera from L. destructor-sensitized subjects, respectively.

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Documento generato il 26/09/20 alle ore 05:40:15