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Titolo:
XMSX-1 MODIFIES MESODERMAL TISSUE PATTERN ALONG DORSOVENTRAL AXIS IN XENOPUS-LAEVIS EMBRYO
Autore:
MAEDA R; KOBAYASHI A; SEKINE R; LIN JJ; KUNG HF; MAENO M;
Indirizzi:
NIIGATA UNIV,FAC SCI,DEPT BIOL,8050 IKARASHI 2 NIIGATA 95021 JAPAN NIIGATA UNIV,FAC SCI,DEPT BIOL NIIGATA 95021 JAPAN NCI,LAB BIOCHEM PHYSIOL,DEPT BASIC SCI,FREDERICK CANC RES & DEV CTR FREDERICK MD 21702
Titolo Testata:
Development
fascicolo: 13, volume: 124, anno: 1997,
pagine: 2553 - 2560
SICI:
0950-1991(1997)124:13<2553:XMMTPA>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
BONE MORPHOGENETIC PROTEIN-4; ANIMAL POLE TISSUE; VENTRAL MESODERM; SPEMANN ORGANIZER; HOMEOBOX GENE; EXPRESSION CLONING; MARGINAL ZONE; LIMB BUDS; INDUCTION; BMP-4;
Keywords:
XMSX-1 RNA; XENOPUS; DORSOVENTRAL AXIS; MESODERM; DORSALISATION; VENTRALISATION; BMP-4;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
59
Recensione:
Indirizzi per estratti:
Citazione:
R. Maeda et al., "XMSX-1 MODIFIES MESODERMAL TISSUE PATTERN ALONG DORSOVENTRAL AXIS IN XENOPUS-LAEVIS EMBRYO", Development, 124(13), 1997, pp. 2553-2560

Abstract

This study analyzes the expression and the function of Xenopus msx-1 (Xmsx-1) in embryos, in relation to the ventralizing activity of bone morphogenetic protein-4 (BMP-4), Expression of Xmsx-1 was increased inUV-treated ventralized embryos and decreased in LiCl-treated dorsalized embryos at the neurula stage (stage 14). Whole-mount in situ hybridization analysis showed that Xmsx-1 is expressed in marginal zone and animal pole areas, laterally and ventrally, but not dorsally, at mid-gastrula (stage 11) and late-gastrula (stage 13) stages. Injection of BMP-4 RNA, but not activin RNA, induced Xmsx-1 expression in the dorsalmarginal zone at the early gastrula stage (stage 10+), and introduction of a dominant negative form of BMP-4 receptor RNA suppressed Xmsx-1expression in animal cap and ventral marginal zone explants at stage 14. Thus, Xmsx-1 is a target gene specifically regulated by BMP-4 signaling. Embryos injected with Xmsx-1 RNA in dorsal blastomeres at the 4-cell stage exhibited a ventralized phenotype, with microcephaly and swollen abdomen. Histological observation and immunostaining revealed that these embryos had a large block of muscle tissue in the dorsal mesodermal area instead of notochord. On the basis of molecular marker analysis, however, the injection of Xmsx-1 RNA did not induce the expression of alpha-globin, nor reduce cardiac alpha-actin in dorsal marginal zone explants. Furthermore, a significant amount of alpha-actin was induced and alpha-globin was turned off in the ventral marginal zone explants injected with Xmsx-1. These results indicated that Xmsx-1 is atarget gene of BMP-4 signaling, but possesses a distinct activity on dorsal-ventral patterning of mesodermal tissues.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/01/20 alle ore 06:27:44