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Titolo:
Inhibition of cyclooxygenase by indomethacin modulates osteoblast responseto titanium surface roughness in a time-dependent manner
Autore:
Sisk, MA; Lohmann, CH; Cochran, DL; Sylvia, VL; Simpson, JP; Dean, DD; Boyan, BD; Schwartz, Z;
Indirizzi:
Univ Texas, Hlth Sci Ctr, Dept Orthopaed, San Antonio, TX 78229 USA Univ Texas San Antonio TX USA 78229 Orthopaed, San Antonio, TX 78229 USA Willard Hall, Med Ctr, San Antonio, TX USA Willard Hall San Antonio TX USA llard Hall, Med Ctr, San Antonio, TX USA Univ Gottingen, Dept Orthopaed, D-3400 Gottingen, Germany Univ Gottingen Gottingen Germany D-3400 opaed, D-3400 Gottingen, Germany Univ Texas, Hlth Sci Ctr, Dept Periodont, San Antonio, TX 78229 USA Univ Texas San Antonio TX USA 78229 Periodont, San Antonio, TX 78229 USA Inst Straumann AG, Waldenburg, Switzerland Inst Straumann AG Waldenburg Switzerland nn AG, Waldenburg, Switzerland Univ Texas, Hlth Sci Ctr, Dept Biochem, San Antonio, TX 78229 USA Univ Texas San Antonio TX USA 78229 pt Biochem, San Antonio, TX 78229 USA Hebrew Univ Jerusalem, Fac Med Dent, Dept Periodont, Jerusalem, Israel Hebrew Univ Jerusalem Jerusalem Israel ept Periodont, Jerusalem, Israel
Titolo Testata:
CLINICAL ORAL IMPLANTS RESEARCH
fascicolo: 1, volume: 12, anno: 2001,
pagine: 52 - 61
SICI:
0905-7161(200102)12:1<52:IOCBIM>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
NONSTEROIDAL ANTIINFLAMMATORY DRUGS; GROWTH-FACTOR-BETA; TGF-BETA; BONE; CELLS; RAT; PROTEIN; MG63; PROSTAGLANDIN-E2; MG-63;
Keywords:
osteoblasts; indomethacin; cyclooxygenase; titanium; surface roughness; differentiation; PGE(2); TGF-beta(1); in vitro;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Boyan, BD Univ Texas, Hlth Sci Ctr, Dept Orthopaed, Mail Code 7774,7703 Floyd Curl Dr, San Antonio, TX 78229 USA Univ Texas Mail Code 7774,7703 FloydCurl Dr San Antonio TX USA 78229
Citazione:
M.A. Sisk et al., "Inhibition of cyclooxygenase by indomethacin modulates osteoblast responseto titanium surface roughness in a time-dependent manner", CLIN OR IMP, 12(1), 2001, pp. 52-61

Abstract

Prostaglandin E-2 (PGE(2)) and transforming growth factor-beta1 (TGF-beta (1)) production are increased in cultures of osteoblasts grown on rough surfaces and prostaglandins are involved in osteoblast response to surface roughness. In the present study, we examined the effect of inhibiting cyclooxygenase on this response. MG63 osteoblast-like cells were cultured on cpTi disks with R-a values of 0.60 mum (PT), 3.97 mum (SLA), and 5.21 mum (TPS) in the presence or absence of 10(-7) M indomethacin. Treatment was begun on days 1, 2, 3, or 4 after seeding, and all cultures were harvested on day 5. Indomethacin decreased PGE(2) release by the cells to less than 50% of basal levels when the cells were cultured on plastic. Cell number decreased with increasing surface roughness and indomethacin treatment abrogated the surface roughness effect over time. Alkaline phosphatase specific activity (ALP) increased with surface roughness; after one day with indomethacin, ALP was decreased on smooth surfaces, but increased on rough surfaces. Over time, ALP decreased on all surfaces examined and remained greater than plasticonly in cultures on TPS. Indomethacin also caused a time-dependent decrease in osteocalcin production on rough surfaces, eventually abrogating the increases due to surface roughness, but had no effect on osteocalcin production on smooth surfaces. TGF-beta (1) levels in the cell layer and media weresensitive to surface roughness; on rougher indomethacin causes a time-dependent abrogation of the response, but has no effect on proliferation, osteocalcin release, or TGF-beta (1) levels on smooth surfaces. surfaces, TCF-beta (1) shifted from the media to the matrix. Indomethacin reduced TGF-beta (1) levels over time, but the surface roughness effect was still evident at4 days. This indicates that prostaglandin production mediates the effects of surface roughness, since indomethacin causes a time-dependent abrogationof the response, but has no effect on profileration, osteocalin release, or TGF-beta (1) levels on smooth surfaces. Indomethacin's effect was not immediate, suggesting that clinical protocols could be designed that would reduce inflammation without preventing osteoblastic differentiation. The effect of indomethacin was not complete, since TCF-beta (1) and ALP remained elevated on rough surfaces, suggesting that pathways or factors other than prostanoids are involved. TCF-beta (1) is preferentially stored in the matrix,acting on the cells through autocrine signaling, and may contribute to ALPeven in the presence of indomethacin. These results demonstrate the importance of local factors in the autocrine regulation of osteogenesis and the potential for factors released in response to surface morphology to act in aparacrine manner.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 12/07/20 alle ore 09:08:12