Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Differential effects of adenovirus-p16 on bladder cancer cell lines can beovercome by the addition of butyrate
Autore:
Lee, CT; Seol, JY; Park, KH; Yoo, CG; Kim, YW; Ahn, C; Song, YW; Han, SK; Han, JS; Kim, S; Lee, JS; Shim, YS;
Indirizzi:
Seoul Natl Univ, Coll Med, Dept Internal Med, Chongno Gu, Seoul 110744, South Korea Seoul Natl Univ Seoul South Korea 110744 o Gu, Seoul 110744, South Korea Seoul Natl Univ, Coll Med, Lung Inst, Med Res Ctr,Chongno Gu, Seoul 110744, South Korea Seoul Natl Univ Seoul South Korea 110744 o Gu, Seoul 110744, South Korea Seoul Natl Univ Hosp, Gene Therapy Lab, Clin Res Inst, Chongno Gu, Seoul 110744, South Korea Seoul Natl Univ Hosp Seoul South Korea 110744 Seoul 110744, South Korea
Titolo Testata:
CLINICAL CANCER RESEARCH
fascicolo: 1, volume: 7, anno: 2001,
pagine: 210 - 214
SICI:
1078-0432(200101)7:1<210:DEOAOB>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
URINARY-BLADDER; GENE-TRANSFER; EXPRESSION; TUMORS; P16;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
18
Recensione:
Indirizzi per estratti:
Indirizzo: Lee, CT Seoul Natl Univ, Coll Med, Dept Internal Med, Chongno Gu, 28 Yongon Dong, Seoul 110744, South Korea Seoul Natl Univ 28 Yongon Dong Seoul South Korea 110744 uth Korea
Citazione:
C.T. Lee et al., "Differential effects of adenovirus-p16 on bladder cancer cell lines can beovercome by the addition of butyrate", CLIN CANC R, 7(1), 2001, pp. 210-214

Abstract

High frequency of p16 alteration and high local recurrence rate of bladdercancer make this cancer an ideal target for pld gene therapy. However, a low transduction rate of p16 via adenoviral vector causes an inconsistent result. In this study, we have tested adenovirus-p16 in several bladder cancer cell lines and investigated a way of improving the low transduction rate. Adenovirus-p16 showed a strong antitumor effect on bladder cancer cell lines (253J and T24) with strong Coxackie-adenoviral receptor (CAR) expressionbut little antitumor effect on bladder cancer cell lines (J82 and HT1376) with little CAR expression. In this study, we suggest a simple way of overcoming the differential effects of the adenovirus, The addition of butyrate to media was found to increase the transduction rate of adenovirus remarkably and increase the antitumor effect of adenovirus-p16 in bladder cancer cell lines with little CAR expression. Butyrate effects were related with increased CAR expression on the cell surface as well as increased transgene expression from adenoviral vector, From these observations, application of adenovirus-pld gene therapy with butyrate can overcome the obstacle of low gene transfer and enhance the antitumor effect of adenovirus-p16 in bladder cancer.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 20:04:35