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Titolo:
Acutely sodded expanded polytetrafluoroethylene grafts produce only prostacyclin: A qualitative difference from saphenous veins
Autore:
Desai, SB; Cable, DG; Phillips, MR; Schaff, HV;
Indirizzi:
Mayo Clin & Mayo Fdn, Cardiovasc Surg Sect, Rochester, MN 55905 USA Mayo Clin & Mayo Fdn Rochester MN USA 55905 Sect, Rochester, MN 55905 USA
Titolo Testata:
CELL TRANSPLANTATION
fascicolo: 6, volume: 9, anno: 2000,
pagine: 797 - 804
SICI:
0963-6897(200011/12)9:6<797:ASEPGP>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
ENDOTHELIUM-DEPENDENT RELAXATION; VASCULAR GRAFTS; SMALL-DIAMETER; CELLS; PATENCY; DACRON; PROSTHESES; SURFACES; REPERFUSION; RECOVERY;
Keywords:
antiplatelet therapy; prostacyclin; saphenous vein; sodded prosthetic graft;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Schaff, HV Mayo Clin & Mayo Fdn, Cardiovasc Surg Sect, 200 1st St SW, Rochester, MN 55905 USA Mayo Clin & Mayo Fdn 200 1st St SW Rochester MN USA 55905 5 USA
Citazione:
S.B. Desai et al., "Acutely sodded expanded polytetrafluoroethylene grafts produce only prostacyclin: A qualitative difference from saphenous veins", CELL TRANSP, 9(6), 2000, pp. 797-804

Abstract

Select subsets of patients require prosthetic graft material fur revascularization. Although arterial prosthetic grafts of large caliber perform acceptably, grafts of <6 mm exhibit a high attrition rate. Microvessel endothelial sodding, a method resulting in the lining of prosthetic grafts with autologous endothelium, improves graft patency; however, aggressive antiplatelet therapy is still required, because terminating an antiplatelet regimen accelerates graft attrition. The present investigation was designed to address the acute production of vasoactive substances in microvessel endothelialcell sodded expanded polytetrafluoroethylene (ePTFE) grafts in an attempt to delineate a possible mechanism behind the continued requirement for antiplatelet therapy. Equal lengths of acutely sodded ePTFE grafts (canine falciform ligament source) and saphenous veins (SV) (canine source) were evaluated by superfusion bioassay. Basal secretion from ePTFE grafts relaxed the biodetector ring 1 +/- 3%, whereas SV relaxed the ring 10 +/- 3% (p < 0.05,ePTFE vs. SV). Relaxation with acetylcholine stimulation was 49 +/- 7% in grafts and 50 +/- 10% in veins (p = NS). Calcium ionophore stimulation produced relaxation of 37 +/- 9% from ePTFE grafts and 100 +/- 23% from SV (p <0.05). Indomethacin added to perfusate reduced relaxations from sodded ePTFE grafts to 20.2 +/- 9.2% with acetylcholine stimulation and 12.5 +/- 4.3%with calcium ionophore (p < 0.05 vs. control): addition of N-G-monomethyl-L-arginine (L-NMMA) had no effect on the release of vasoactive substances from ePTFE grafts. In contrast, relaxations of effluent from SV stimulated by acetylcholine and calcium ionophore were significantly attenuated with indomethacin and L-NMMA (p < 0.05 vs. control). Scanning electron microscopy demonstrated confluent endothelium in SV and a nonconfluent endothelial cell layer in grafts. Acutely sodded ePTFE grafts produce vasoactive substances that quantitatively and qualitatively differ from those produced by canine SV. The ePTFE grafts produce mainly prostanoids, whereas SV produce both nitric oxide and prostanoids. The endothelial cell isolation procedure and absence of immediate graft luminal confluence may contribute to the observed differences.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/21 alle ore 12:22:10