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Titolo:
Differences in early and late responses between neurotrophin-stimulated trkA- and trkC-transfected SH-SY5Y neuroblastoma cells
Autore:
Edsjo, A; Hallberg, B; Fagerstrom, S; Larsson, C; Axelson, H; Pahlman, S;
Indirizzi:
Univ Lund, Univ Hosp MAS, Dept Lab Med, Div Mol Med, S-20502 Malmo, SwedenUniv Lund Malmo Sweden S-20502 b Med, Div Mol Med, S-20502 Malmo, Sweden Umea Univ, Dept Cell & Mol Biol, S-90187 Umea, Sweden Umea Univ Umea Sweden S-90187 Dept Cell & Mol Biol, S-90187 Umea, Sweden
Titolo Testata:
CELL GROWTH & DIFFERENTIATION
fascicolo: 1, volume: 12, anno: 2001,
pagine: 39 - 50
SICI:
1044-9523(200101)12:1<39:DIEALR>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
NERVE GROWTH-FACTOR; SYMPATHETIC NEUROBLASTS; NEURONAL DIFFERENTIATION; IN-VIVO; PROTOONCOGENE PRODUCT; RECEPTOR EXPRESSION; SURVIVAL; PROTEIN; GENE; KINASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
57
Recensione:
Indirizzi per estratti:
Indirizzo: Pahlman, S Univ Lund, Univ Hosp MAS, Dept Lab Med, Div Mol Med, Entrance 78, S-20502 Malmo, Sweden Univ Lund Entrance 78 Malmo Sweden S-20502 20502 Malmo, Sweden
Citazione:
A. Edsjo et al., "Differences in early and late responses between neurotrophin-stimulated trkA- and trkC-transfected SH-SY5Y neuroblastoma cells", CELL GROWTH, 12(1), 2001, pp. 39-50

Abstract

Despite their sympathetic neuroblast origin, highly malignant neuroblastoma tumors and derived cell lines have no or low expression of the neurotrophin receptor genes, trkA and trkC. Expression of exogenous trkA in neuroblastoma cells restores their ability to differentiate in response to nerve growth factor (NGF), Here we show that stable expression of trkC in SH-SY5Y neuroblastoma cells resulted in morphological and biochemical differentiationupon treatment with neurotrophin-3 (NT-3), To some extent, trkA- and trkC-transfected SH-SY5Y (SH-SY5Y/trkA and SH-SY5Y/trkC) cells resembled one another in terms of early signaling events and neuronal marker gene expression, but important differences were observed. Although induced Erk 1/2 and Akt/PKB phosphorylation was stronger in NT-3-stimulated SH-Y5Y/trkC cells, activation of the immediate-early genes tested was more prominent in NGF-treated SH-SY5Y/trkA cells. In particular, c-fos was not induced in the SH-SY5Y/trkC cells. There were also phenotypic differences. The concentrations of norepinephrine, the major sympathetic neurotransmitter, and growth cone-located synaptophysin, a neurosecretory granule protein, were increased in NGF-treated SH-SY5Y/trkA but not in NT-3-treated SH-SY5Y/trkC cells. Our data suggest that NT-3/p145(trkC) and NGF/p140(trkA) signaling differ in some aspects in neuroblasoma cells, and that this may explain the phenotypic differences seen in the long-term neurotrophin-treated cells.

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Documento generato il 21/09/20 alle ore 16:18:20