Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Establishment and characterization of a neu xenograft-derived human esophageal squamous cell carcinoma cell line SLMT-1 of Chinese origin
Autore:
Tang, JCO; Wan, TSK; Wong, N; Pang, E; Lam, KY; Law, SY; Chow, LMC; Ma, ESK; Chan, LC; Wong, J; Srivastava, G;
Indirizzi:
Univ Hong Kong, Dept Pathol, Hong Kong, Hong Kong, Peoples R China Univ Hong Kong Hong Kong Hong Kong Peoples R China Kong, Peoples R China Hong Kong Polytech Univ, Dept Appl Biol & Chem Technol, Hong Kong, Hong Kong, Peoples R China Hong Kong Polytech Univ Hong Kong Hong Kong Peoples R China ples R China Chinese Univ Hong Kong, Dept Clin Oncol, Sir YK Pao Ctr Canc, Hong Kong, Hong Kong, Peoples R China Chinese Univ Hong Kong Hong Kong Hong Kong Peoples R China oples R China Univ Hong Kong, Dept Surg, Hong Kong, Hong Kong, Peoples R China Univ HongKong Hong Kong Hong Kong Peoples R China Kong, Peoples R China
Titolo Testata:
CANCER GENETICS AND CYTOGENETICS
fascicolo: 1, volume: 124, anno: 2001,
pagine: 36 - 41
SICI:
0165-4608(20010101)124:1<36:EACOAN>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
COMPARATIVE GENOMIC HYBRIDIZATION; INVOLVEMENT; ONCOGENES; GAIN; GENE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
21
Recensione:
Indirizzi per estratti:
Indirizzo: Srivastava, G Univ Hong Kong, Dept Pathol, Hong Kong, Hong Kong, Peoples RChina Univ Hong Kong Hong Kong Hong Kong Peoples R China R China
Citazione:
J.C.O. Tang et al., "Establishment and characterization of a neu xenograft-derived human esophageal squamous cell carcinoma cell line SLMT-1 of Chinese origin", CANC GENET, 124(1), 2001, pp. 36-41

Abstract

A new human esophageal cancer cell line, named SLMT-1,was established froma nude-mouse xenograft of a well-differentiated esophageal squamous cell carcinoma (ESCC) of the lower esophagus from a male Hong Kong Chinese patient. SLMT-1, passaged over 34 times and with a doubling time of 31 hours, hasthe microscopic features of epithelial cells with adherent growth as a mono-layer. The general biologic properties of SLMT-1 cells were characterizedby (1) a positive test of tumorigenicity obtained by injecting cells subcutaneously into athymic nude mice and observing their development into well-differentiated squamous cell carcinoma; (2) immunohistochemical staining using antibodies (AE1/AE3, CAM5.2 and MAK 6) which show the presence of cytokeratin intermediate filaments; and (3) electron microscopy demonstrating the morphologic features of epithelial cells with the presence of desmosomes. The cytogenetic abnormalities found in both the primary culture and SLMT-1included der(1;14)(q10;q10). add(1)(p1?), +1, c2, del(3)(q11), +6, +7, i(g)(q10), +8, +10, +11, -13, -15, 1-16. +17, -18, -19, -Y and marker chromosomes. Additional changes observed in the 34th passage included gains as wellas losses of both numerical and structural abnormalities. Comparative genomic hybridization (CGH) indicated copy number gains on chromosomal regions 3q32-qter, 5p, Sp:12-p11.2, 11q13-q22 and 13q22-qter, and loss of the Y. The gains of 8p12-p11.2 in SLMT-1 cells are novel to ESCC. Based on its distinct and common characteristics, the SLMT-1 cell line serves as a useful tool for studying the molecular and genetic basis of the pathogenesis of ESCC. (C) 2001 Elsevier Science Inc. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 02:16:53