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Titolo:
Experimental study of a type 3 phosphodiesterase inhibitor on liver graft function
Autore:
Ikegami, T; Nishizaki, T; Hiroshige, S; Ohta, R; Yanaga, K; Sugimachi, K;
Indirizzi:
Kyushu Univ, Dept Surg 2, Fukuoka 8128582, Japan Kyushu Univ Fukuoka Japan 8128582 v, Dept Surg 2, Fukuoka 8128582, Japan
Titolo Testata:
BRITISH JOURNAL OF SURGERY
fascicolo: 1, volume: 88, anno: 2001,
pagine: 59 - 64
SICI:
0007-1323(200101)88:1<59:ESOAT3>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEART-BEATING DONORS; CYCLIC-NUCLEOTIDE PHOSPHODIESTERASES; TRANSPLANTATION; RAT; AMRINONE; AMP; INJURY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
22
Recensione:
Indirizzi per estratti:
Indirizzo: Ikegami, T Kyushu Univ, Dept Surg 2, Fukuoka 8128582, Japan Kyushu Univ Fukuoka Japan 8128582 g 2, Fukuoka 8128582, Japan
Citazione:
T. Ikegami et al., "Experimental study of a type 3 phosphodiesterase inhibitor on liver graft function", BR J SURG, 88(1), 2001, pp. 59-64

Abstract

Background: The number of liver transplant recipients is increasing but donor organ shortages have become more severe. The effect of milrinone, a type 3 phosphodiesterase inhibitor (PDEI), on non-heart-beating donor grafts was evaluated using an orthotopic liver transplantation model in rats. Methods: Type 3 PDEI or normal saline (control group) was given intravenously to the donor animals for 60 min continuously (50 mu gkg(-1)min(-1)) before 60 min of warm ischaemia followed by cold preservation and subsequent transplantation Survival, serum chemistry, bile output, histopathological findings and tissue cyclic 3',5'-adenosine monophosphate (cAMP) concentrations were then compared. Results: Five of seven animals in the PDEI group were alive at 7 days, compared with only one of seven rats in the control group (P < 0.01). Serum levels of alanine aminotransferase 2 and 6 h after reperfusion, and hyaluronic acid levels 6 h after reperfusion, were significantly lower in the PDEI group than in the control group. Bile output from the transplanted graft wassignificantly greater in the PDEI group than in controls 2 h after reperfusion (P < 0.01). The mean necrotic area 6 h after reperfusion was also reduced in the PDEI-treated grafts (P < 0.01). cAMP levels in liver tissue at the end of both warm and cold ischaemia, and 2 and 6 h after reperfusion, were significantly higher in the PDEI group compared with those in the control group. Conclusion: Type 3 PDEI attenuated the graft injury caused by warm and cold ischaemia and subsequent reperfusion injury via an increase in intracellular cAMP levels. This treatment may be a novel pharmacological interventionfor safe and efficient usage of liver grafts from non-heart-beating donors.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/04/20 alle ore 11:19:52