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Titolo:
The effect of superoxide dismutase on nitric oxide-mediated and electricalfield-stimulated diabetic rabbit cavernosal smooth muscle relaxation
Autore:
Khan, MA; Thompson, CS; Jeremy, JY; Mumtaz, FH; Mikhailidis, P; Morgan, RJ;
Indirizzi:
Royal Free Campus & Royal Free Hampstead NHS Trus, Univ Coll London, RoyalFree & Univ Coll Med Sch, Dept Urol, London, England Royal Free Campus & Royal Free Hampstead NHS Trus London England ngland Royal Free Campus & Royal Free Hampstead NHS Trus, Univ Coll London, RoyalFree & Univ Coll Med Sch, Dept Mol Pathol & Clin Biochem, London, England Royal Free Campus & Royal Free Hampstead NHS Trus London England ngland Bristol Royal Infirm, Dept Cardiac Surg, Bristol, Avon, England Bristol Royal Infirm Bristol Avon England c Surg, Bristol, Avon, England
Titolo Testata:
BJU INTERNATIONAL
fascicolo: 1, volume: 87, anno: 2001,
pagine: 98 - 103
SICI:
1464-4096(200101)87:1<98:TEOSDO>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
ENDOTHELIUM-DEPENDENT RELAXATION; CORPUS CAVERNOSUM; IMPOTENT MEN; MELLITUS; PEROXYNITRITE; DYSFUNCTION; PENIS; ANION; RATS;
Keywords:
rabbit; diabetes mellitus; erectile dysfunction; superoxide dismutase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Morgan, RJ Royal Free Hosp, Dept Urol, Pond St, London NW3 2QG, England Royal Free Hosp Pond St London England NW3 2QG W3 2QG, England
Citazione:
M.A. Khan et al., "The effect of superoxide dismutase on nitric oxide-mediated and electricalfield-stimulated diabetic rabbit cavernosal smooth muscle relaxation", BJU INT, 87(1), 2001, pp. 98-103

Abstract

Objective To investigate the effect of superoxide dismutase (SOD, the enzyme that accelerates the breakdown of the superoxide anion, O-2(-) to H2O) on nitric oxide (NO)-mediated and electrical field stimulated (EFS) relaxation in diabetic rabbit cavernosal smooth muscle. Materials and methods Diabetes was induced with alloxan (65 mg/kg) in six adult New Zealand White rabbits. After 6 months, cavernosal smooth muscle strips from age-matched controls and diabetic animals were mounted in organ baths. After precontraction with phenylephrine (10 mu mol/L) in the presence of atropine (1 mu mol/L), guanethidine (5 mu Lmol/L) and indomethacin (10mu mol/L), relaxation responses to EFS (1-20 Hz), carbachol (10(-8)-10(-4)mol/L) and sodium nitroprusside (SNP, 10(-9)-10(-4) mol/L) were assessed in the presence and absence of SOD (100 IU/mL). Results SNP- and carbachol-mediated (endothelium-independent and -dependent, respectively) relaxations were impaired in the diabetic cavernosal smooth muscle strips compared with controls (concentration required for 50% inhibition, 1.4 mu mol/L for diabetic and 0.75 mu mol/L for control with SNP, and 44 mu mol/L for diabetic and 0.4 mu mol/L for control with carbachol). SOD significantly enhanced both SNP- and carbachol-mediated diabetic cavernosal smooth muscle relaxations (both P < 0.05). EFS-mediated relaxations were also significantly (P < 0.05) impaired in the diabetic cavernosal smooth muscle strips; these relaxations were also significantly (P < 0.05) enhanced by SOD. Conclusion NO- and EFS-mediated cavernosal smooth muscle relaxation is impaired in a rabbit model of diabetes but SOD significantly reversed the impaired relaxation. Therefore, in diabetes, the generation of reactive oxygen species may play an important role in the development of erectile dysfunction.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/04/20 alle ore 07:06:00