Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Preparation and properties of Clostridium thermocellum lichenase deletion variants and their use for construction of bifunctional hybrid proteins
Autore:
Musiychuk, KA; Goldenkova, IV; Abdeev, RM; Kobets, NS; Piruzian, ES;
Indirizzi:
Russian Acad Sci, Vavilov Inst Gen Genet, Moscow 117809, Russia Russian Acad Sci Moscow Russia 117809 t Gen Genet, Moscow 117809, Russia
Titolo Testata:
BIOCHEMISTRY-MOSCOW
fascicolo: 12, volume: 65, anno: 2000,
pagine: 1397 - 1402
SICI:
0006-2979(200012)65:12<1397:PAPOCT>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
GREEN FLUORESCENT PROTEIN; BETA-GLUCURONIDASE; TRANSGENIC PLANTS; ESCHERICHIA-COLI; GENE-EXPRESSION; REPORTER GENE; CELLS; CELLULASES; XYLANASE; DOMAINS;
Keywords:
thermostable lichenase (beta-1,3-1,4-glucanase); green fluorescent protein (GFP); reporter systems; hybrid bifunctional proteins;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Musiychuk, KA Russian Acad Sci, Vavilov Inst Gen Genet, Ul Gubkina 3, Moscow 117809, Russia Russian Acad Sci Ul Gubkina 3 Moscow Russia 117809 , Russia
Citazione:
K.A. Musiychuk et al., "Preparation and properties of Clostridium thermocellum lichenase deletion variants and their use for construction of bifunctional hybrid proteins", BIOCHEM-MOS, 65(12), 2000, pp. 1397-1402

Abstract

Major properties (pH and temperature optimum, stability) of lichenase (beta -1,3-1,4-glucanase) deletion variants from Clostridium thermocellum were comparatively studied. The deletion variant LicBM2 was used to create hybrid bifunctional proteins by fusion with sequences of the green fluorescent protein (GFP) from Aequorea victoria. The data show that in hybrid proteins both GFP and lichenase retain their major properties, namely, GFP remains afluorescent protein and the lichenase retains activity and high thermostability. Based on the results of this investigation and results that have been obtained earlier, the use of the deletion variants of lichenase and the bifunctional hybrid proteins as reporter proteins is suggested.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 05:36:55