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Titolo:
Molecular cloning, expression, and regulation of hippocampal amyloid precursor protein of senescence accelerated mouse (SAMP8)
Autore:
Kumar, VB; Vyas, K; Franko, M; Choudhary, V; Buddhiraju, C; Alvarez, J; Morley, JE;
Indirizzi:
Vet Adm Med Ctr, Ctr Geriatr Res Educ & Clin, St Louis, MO 63125 USA Vet Adm Med Ctr St Louis MO USA 63125 Educ & Clin, St Louis, MO 63125 USA St Louis Univ, Div Geriatr Med, St Louis, MO 63125 USA St Louis Univ St Louis MO USA 63125 v Geriatr Med, St Louis, MO 63125 USA
Titolo Testata:
BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE
fascicolo: 1, volume: 79, anno: 2001,
pagine: 57 - 67
SICI:
0829-8211(200102)79:1<57:MCEARO>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
FAMILIAL ALZHEIMERS-DISEASE; MESSENGER-RNA; ANTISENSE OLIGONUCLEOTIDES; NEUROFIBRILLARY TANGLES; SELECTIVE-INHIBITION; HAMMERHEAD RIBOZYME; COMPLEMENTARY-DNA; RIBONUCLEIC-ACID; GENE-EXPRESSION; DOWN-REGULATION;
Keywords:
cloning; amyloid precursor protein; transfection; expression; antisense oligo;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
51
Recensione:
Indirizzi per estratti:
Indirizzo: Kumar, VB Vet Adm Med Ctr, Ctr Geriatr Res Educ & Clin, St Louis, MO 63125USA Vet Adm Med Ctr St Louis MO USA 63125 n, St Louis, MO 63125 USA
Citazione:
V.B. Kumar et al., "Molecular cloning, expression, and regulation of hippocampal amyloid precursor protein of senescence accelerated mouse (SAMP8)", BIOC CELL B, 79(1), 2001, pp. 57-67

Abstract

Alzheimer's disease (AD) is associated with increased expression of amyloid precursor protein (APP) with a consequent deposition of amyloid beta peptide (A beta) which forms characteristic senile plaques. We have noticed that the senescence accelerated mouse (SAMP8), a strain of mouse that exhibitsage-dependent defects such as loss of memory and retention at an early ageof 8-12 months, also produces increased amounts of APP and A beta similar to those observed in Alzheimer's disease (AD). In order to investigate if this is due to mutations in APP similar to those observed in AD, and to develop molecular probes that regulate its expression, APP cDNA was cloned fromthe hippocampus of 8-month-old SAMP8 mouse. The nucleotide sequence is 99.7% homologous with that of mouse and rat, 88.7% with monkey, and 89.2% withhuman homologues. At the amino acid level, the homology was 99.2% and 97.6% with rodent and primate sequences, respectively. A single amino acid substitution of Alanine instead of Valine at position 300 was unique to SAMP8 mouse APP. However, no mutations similar to those reported in human familialAD were observed. When the cDNA was expressed in HeLa cells, glycosylated mature APP could be detected by immunoblotting technique. The expression could be regulated in a time- and concentration-dependent manner by using an antisense oligonucleotide specific to APP mRNA. Such regulation of APP expression may have a therapeutic application in vivo.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/01/20 alle ore 22:15:26