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Titolo:
On-line monitoring and controlling system for fermentation processes
Autore:
Liu, YC; Wang, FS; Lee, WC;
Indirizzi:
Natl Chung Cheng Univ, Dept Chem Engn, Chiayi 62107, Taiwan Natl Chung Cheng Univ Chiayi Taiwan 62107 hem Engn, Chiayi 62107, Taiwan
Titolo Testata:
BIOCHEMICAL ENGINEERING JOURNAL
fascicolo: 1, volume: 7, anno: 2001,
pagine: 17 - 25
SICI:
1369-703X(200101)7:1<17:OMACSF>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
PERFORMANCE LIQUID-CHROMATOGRAPHY; ZYMOMONAS-MOBILIS; ETHANOL-PRODUCTION; ALCOHOL PRODUCTION; ESCHERICHIA-COLI; ACID-PRODUCTION; ONLINE; CULTURES; HPLC;
Keywords:
bioprocess monitoring; control; ethanol; fermentation; sensors; chromatography;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
18
Recensione:
Indirizzi per estratti:
Indirizzo: Wang, FS Natl Chung Cheng Univ, Dept Chem Engn, Chiayi 62107, Taiwan Natl Chung Cheng Univ Chiayi Taiwan 62107 Chiayi 62107, Taiwan
Citazione:
Y.C. Liu et al., "On-line monitoring and controlling system for fermentation processes", BIOCH ENG J, 7(1), 2001, pp. 17-25

Abstract

A personal computer-based on-line monitoring and controlling system was developed for the fermentation of microorganism. The on-line HPLC system for the analysis of glucose and ethanol in the fermentation broth was connectedto the fermenter via an auto-sampling equipment, which could perform the pipetting, filtration and dilution of the sample and final injection onto the HPLC through automation based on a programmed procedure. The A/D and D/A interfaces were equipped in order to process the signals from electrodes and from the detector of HPLC, and to direct the feed pumps, the motor of stirrer and gas flow-rate controller. The software that supervised the controlof the stirring speed, gas flow-rate, pH value, feed flow-rate of medium, and the on-line measurement of glucose and ethanol concentration was programmed by using Microsoft Visual Basic under Microsoft Windows. The signal for chromatographic peaks from on-line HPLC was well captured and processed using an RC filter and a smoothing algorithm. This monitoring and control system was demonstrated to be effective in the ethanol fermentation of Zymomonas mobilis operated in both batch and fed-batch modes. In addition to substrate and product concentrations determined by on line HPLC, the biomass concentration in Z. mobilis fermentation could also be on-line estimated by using the pH control and an implemented software sensor. The substrate concentration profile in the fed-back fermentation followed well the set point profile due to the fed-back action of feed flow-rate control. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/04/20 alle ore 17:43:48