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Titolo:
A lectin immunosensor technique for determination of alpha(1)-acid glycoprotein fucosylation
Autore:
Liljeblad, M; Ryden, I; Ohlson, S; Lundblad, A; Pahlsson, P;
Indirizzi:
Linkoping Univ, Dept Biomed & Surg, Div Clin Chem, SE-58185 Linkoping, Sweden Linkoping Univ Linkoping Sweden SE-58185 hem, SE-58185 Linkoping, Sweden Linkoping Univ, Forum Scientum Grad Sch, SE-58185 Linkoping, Sweden Linkoping Univ Linkoping Sweden SE-58185 Sch, SE-58185 Linkoping, Sweden Kalmar Cty Hosp, Dept Clin Chem, SE-39185 Kalmar, Sweden Kalmar Cty Hosp Kalmar Sweden SE-39185 lin Chem, SE-39185 Kalmar, Sweden Univ Kalmar, Dept Chem & Biomed Sci, Div Biochem Biotechnol, SE-39182 Kalmar, Sweden Univ Kalmar Kalmar Sweden SE-39182 m Biotechnol, SE-39182 Kalmar, Sweden
Titolo Testata:
ANALYTICAL BIOCHEMISTRY
fascicolo: 2, volume: 288, anno: 2001,
pagine: 216 - 224
SICI:
0003-2697(20010115)288:2<216:ALITFD>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
SURFACE-PLASMON RESONANCE; BIOSPECIFIC INTERACTION ANALYSIS; ALPHA-1-ACID GLYCOPROTEIN; GLYCOSYLATION; OLIGOSACCHARIDES; PURIFICATION; PROTEINS; AFFINITY; BINDING; DISEASE;
Keywords:
surface plasmon resonance; glycosylation; alpha(1)-acid glycoprotein; Aleuria aurentia lectin; fucose;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
20
Recensione:
Indirizzi per estratti:
Indirizzo: Liljeblad, M Linkoping Univ, Dept Biomed & Surg, Div Clin Chem, SE-58185 Linkoping, Sweden Linkoping Univ Linkoping Sweden SE-58185 Linkoping, Sweden
Citazione:
M. Liljeblad et al., "A lectin immunosensor technique for determination of alpha(1)-acid glycoprotein fucosylation", ANALYT BIOC, 288(2), 2001, pp. 216-224

Abstract

The fucosylation of alpha (1)-acid glycoprotein (AGP), an acute-phase protein, is known to change in association with inflammatory diseases. Thus, fucosylation of AGP could be a potential diagnostic or prognostic marker. Thechange in fucosylation has previously been investigated using crossed affinoimmunoelectrophoresis, high-pa. anion exchange chromatography, and lectinELISA. This study describes a surface plasmon resonance-based affinity biosensor assay for quantification of the fucosylation of AGP. Diluted EDTA plasma or serum was injected directly in a BIACORE 2000 biosensor. AGP was captured on the sensor surface using immobilized antibodies and a fucose-binding lectin from Aleuria aurentia was then used for the detection of fucosylation. The feature of the biosensor makes it possible to determine both theamount of bound AGP and the amount of bound lectin. Using a calibration curve it was possible to obtain a fucosylation ratio that was independent of AGP concentration. The assay was validated against a lectin ELISA and used to follow inflammation in patients with severe burns. (C) 2001 Academic Press.

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Documento generato il 03/04/20 alle ore 09:23:38