Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Calreticulin is an interleukin-3-sensitive calcium-binding protein in human basophil leukocytes
Autore:
Lyngholm, JM; Nielsen, HV; Holm, M; Schiotz, PO; Johnsen, AH;
Indirizzi:
Aarhus Univ Hosp, Dept Pediat, DK-8200 Aarhus N, Denmark Aarhus Univ HospAarhus Denmark N Dept Pediat, DK-8200 Aarhus N, Denmark Natl Univ Hosp, Dept Clin Biochem, Copenhagen, Denmark Natl Univ Hosp Copenhagen Denmark ept Clin Biochem, Copenhagen, Denmark
Titolo Testata:
ALLERGY
fascicolo: 1, volume: 56, anno: 2001,
pagine: 21 - 28
SICI:
0105-4538(200101)56:1<21:CIAICP>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
HISTAMINE-RELEASE; SIGNAL-TRANSDUCTION; MEDIATOR RELEASE; BLOOD BASOPHILS; GENE-EXPRESSION; RECEPTOR; CELLS; IL-3; GENERATION; MODULATION;
Keywords:
basophils; calcium-binding proteins; calreticulin; IL-3;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Nielsen, HV Aarhus Univ Hosp, Dept Pediat, Brendstrupgaardsvej 100, DK-8200 Aarhus N, Denmark Aarhus Univ Hosp Brendstrupgaardsvej 100 Aarhus Denmark N ark
Citazione:
J.M. Lyngholm et al., "Calreticulin is an interleukin-3-sensitive calcium-binding protein in human basophil leukocytes", ALLERGY, 56(1), 2001, pp. 21-28

Abstract

Background: IL-3 Enhances basophil histamine release upon stimulation withany known secretagogue. The molecular mechanism behind this regulation is not known, although some observations suggest that IL-3 modulates the calcium part of the signal transduction mechanism. The inhibitory action of glucocorticoids on basophils can be reversed by stimulation with IL-3. Methods: Calcium-binding proteins in the basophil cell line KU812 were identified by two-dimensional gel electrophoresis, Calcium-overlay assay, N-terminal sequence analysis, and mass spectometry. The presence of the same proteins in purified human basophil leukocytes was established by comigrationof KU812 and human basophil proteins on the two-dimensional gels. The expression of the calcium-binding proteins in the absence and presence of IL-3 and/or anti-IgE was determined by densitometric measurement of the spots onthe two-dimensional gels. Results: Calreticulin was identified on the two-dimensional gel of KU812 proteins. A protein with exactly the same migration pattern was found on thegels of proteins from purified human basophils. Immunoblotting with a specific antihuman calreticulin antibody confirmed that this protein was calreticulin. Subsequent analysis showed that the expression of calreticulin in the basophils is upregulated twofold upon stimulation with rhIL-3, even in doses below those needed for enhancement of histamine release. Conclusions: The expression of calreticulin in human basophil leukocytes is regulated by IL-3. Calreticulin is known to modulate IP3-dependent Ca2+ influx in different cell systems, and calreticulin overexpression inhibits steroid-induced transcriptional activation. Therefore, modulation of calreticulin expression may be one mechanism by which IL-3 exerts its effects on human basophils.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/04/20 alle ore 18:16:36