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Titolo:
Immunogenicity of Ly5 (CD45)-antigens hampers long-term engraftment following minimal conditioning in a murine bone marrow transplantation model
Autore:
van Os, R; Sheridan, TM; Robinson, S; Drukteinis, D; Ferrara, JLM; Mauch, PM;
Indirizzi:
Brigham & Womens Hosp, Dept Radiat Oncol, Boston, MA 02115 USA Brigham & Womens Hosp Boston MA USA 02115 iat Oncol, Boston, MA 02115 USA Harvard Univ, Sch Med, Dana Farber Canc Inst, Boston, MA 02115 USA HarvardUniv Boston MA USA 02115 a Farber Canc Inst, Boston, MA 02115 USA Univ Michigan, Ctr Canc, Combined Bone Marrow Transplantat Program, Ann Arbor, MI 48109 USA Univ Michigan Ann Arbor MI USA 48109 tat Program, Ann Arbor, MI 48109 USA
Titolo Testata:
STEM CELLS
fascicolo: 1, volume: 19, anno: 2001,
pagine: 80 - 87
SICI:
1066-5099(2001)19:1<80:IOL(HL>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEMATOPOIETIC STEM-CELLS; TOTAL-BODY IRRADIATION; DONOR-TYPE CHIMERISM; REPOPULATING ABILITY; LETHAL IRRADIATION; MIXED CHIMERISM; HOST DISEASE; T-CELLS; RECIPIENTS; MICE;
Keywords:
engraftment; Gpi; Ly5; immunity;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: van Os, R Leiden Univ, Med Ctr, Dept Hematol, Bldg 1,C2-R,POB 9600, NL-2300 RC Leiden, Netherlands Leiden Univ Bldg 1,C2-R,POB 9600 Leiden Netherlands NL-2300 RC
Citazione:
R. van Os et al., "Immunogenicity of Ly5 (CD45)-antigens hampers long-term engraftment following minimal conditioning in a murine bone marrow transplantation model", STEM CELLS, 19(1), 2001, pp. 80-87

Abstract

Various techniques are available for distinguishing donor from host cells evaluating the efficacy of conditioning regimen for experimental bone marrow transplantation (BMT). Techniques include the use of extracellular immunological markers, such as Ly5 (CD45), and intracellular biochemical markers,such as glucose-phosphate-isomerase (Gpi), Because Ly5 is an extracellularprotein, the disparity between donor (Ly5.1) and host (Ly5.2) antigens mayinduce a weak immune response whereas with Gpi, no immune response is expected, This difference may be of particular concern in experimental transplantation approaches that use minimal conditioning such as low-dose total body irradiation (TBI), Such mild conditioning mag not induce the immunosuppression required to overcome host rejection of Ly5 disparate cells. To compare the relative engraftment of Lp5.1 and Gpi-1(a) donor marrow, B6(Gpi-1(h)/Ly5.2) mice were irradiated with low-level TBI (0-6 Gy) and transplanted with several hone marrow (BM) doses (2 x 10(6)-5 x 10(7) cells). At 8, 26, and, 52 weeks post-BMT, the level, of donor engraftment was measured using now cytometry (Ly5) or Gpi-electrophoresis,Lower engraftment levels were found in mice transplanted with Ly5 congenicBM in groups given low-dose TBI (less than or equal to 4 Gy) and/or low doses of BM cells (BMC) (2 x 10(6)), However, when higher TBI or BR IC doses were used, similar engraftment levels were found, suggesting sufficient immune suppression to allow equal engraftment of both sources of BM. These data suggest that even a minor phenotypic disparity between donor and host, such as Ly5, may necessitate high-dose TBI to prevent rejection, The combination of low-dose TBI or other nonmyeloablative conditioning strategies with small numbers of BMC may lead to reduced engraftment when extracellular immunological markers such as Ly5 are used for transplantation studies, Therefore, small immunological differences must be considered when using the Ly5 marker for engraftment.

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Documento generato il 05/04/20 alle ore 05:23:55