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Titolo:
Major histocompatibility complex restriction between hematopoietic stem cells and stromal cells in vitro
Autore:
Sugiura, K; Hisha, H; Ishikawa, J; Adachi, Y; Taketani, S; Lee, S; Nagahama, T; Ikehara, S;
Indirizzi:
Kansai Med Univ, Dept Pathol 1, Transplantat Ctr, Moriguchi, Osaka 5708506, Japan Kansai Med Univ Moriguchi Osaka Japan 5708506 guchi, Osaka 5708506, Japan Novartis Pharma, Tukuba Res Inst, Tukuba City, Japan Novartis Pharma Tukuba City Japan , Tukuba Res Inst, Tukuba City, Japan Kansai Med Univ, Dept Hyg, Transplantat Ctr, Moriguchi, Osaka 5708506, Japan Kansai Med Univ Moriguchi Osaka Japan 5708506 guchi, Osaka 5708506, Japan
Titolo Testata:
STEM CELLS
fascicolo: 1, volume: 19, anno: 2001,
pagine: 46 - 58
SICI:
1066-5099(2001)19:1<46:MHCRBH>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
BONE-MARROW TRANSPLANTATION; COLONY-FORMING CELLS; CLASS-I ANTIGENS; GROWTH-FACTOR; PROGENITOR CELLS; SIGNAL-TRANSDUCTION; ACUTE REJECTION; T-CELLS; MICE; ADHESION;
Keywords:
hematopoietic stem cells; stromal cells; restriction; MHC class la; cobblestone colony;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
69
Recensione:
Indirizzi per estratti:
Indirizzo: Ikehara, S Kansai Med Univ, Dept Pathol 1, Transplantat Ctr, 10-15 Fumizono, Moriguchi, Osaka 5708506, Japan Kansai Med Univ 10-15 Fumizono MoriguchiOsaka Japan 5708506 an
Citazione:
K. Sugiura et al., "Major histocompatibility complex restriction between hematopoietic stem cells and stromal cells in vitro", STEM CELLS, 19(1), 2001, pp. 46-58

Abstract

We have previously found that a significant number of hematopoietic progenitors accumulate in engrafted bones with the same major histocompatibility complex (MHC) as the transplanted bone marrow cells. In the present study, to further clarify the MHC restriction between hematopoietic stem cells (HSC) and microenvironment, me carried out cobblestone colony formation assaysby culturing HSCs with MHC-matched or -mismatched stromal cell monolayers. The formation of cobblestone colonies under MHC-mismatched stromal cells significantly decreased in comparison with MHC-matched stromal cells, However, the decrease in cobblestone colony formation under MHC-mismatched stromal cells was not significant when using MHC class I-deficient HSC or stromalcells, Taken together with the results using B10 congenic strains, it is suggested that the MHC preference is restricted by MHC class la molecules. Treatment with monoclonal antibodies (mAbs) against MHC class Ia molecules of stromal cell phenotypes significantly enhanced the cobblestone colony formation, whereas treatment with mAbs against HSC phenotypes significantly inhibited it. The expression of cytokines to promote hematopoiesis was enhanced by the mAbs against stromal cell phenotypes. The enhancement of cytokineexpression was also observed when stromal cells and HSCs were MHC-matchcd,These results suggest that signaling via the MHC molecules augments stromal cell activity and elicits the MHC restriction.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 10:58:56