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Titolo:
New strategies in the treatment of acute myelogenous leukemia (AML): In vitro culture of AML cells - The present use in experimental studies and the possible importance for future therapeutic approaches
Autore:
Bruserud, O; Gjertsen, BT; Foss, B; Huang, TS;
Indirizzi:
Haukeland Univ Hosp, Dept Med, Div Hematol, N-5021 Bergen, Norway Haukeland Univ Hosp Bergen Norway N-5021 Hematol, N-5021 Bergen, Norway Univ Bergen, Inst Mol Biol, Bergen, Norway Univ Bergen Bergen NorwayUniv Bergen, Inst Mol Biol, Bergen, Norway
Titolo Testata:
STEM CELLS
fascicolo: 1, volume: 19, anno: 2001,
pagine: 1 - 11
SICI:
1066-5099(2001)19:1<1:NSITTO>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACUTE MYELOID-LEUKEMIA; ACUTE MYELOBLASTIC-LEUKEMIA; CONSTITUTIVE CYTOKINE SECRETION; COLONY-STIMULATING FACTOR; SERUM-FREE CULTURE; ABILITY IN-VITRO; GROWTH-FACTOR; BLAST CELLS; AUTONOMOUS GROWTH; PROGNOSTIC-SIGNIFICANCE;
Keywords:
acute myelogenous leukemia; experimental models; in vitro culture; chemotherapy; apoptosis;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
75
Recensione:
Indirizzi per estratti:
Indirizzo: Bruserud, O Haukeland Univ Hosp, Dept Med, Div Hematol, N-5021 Bergen, Norway Haukeland Univ Hosp Bergen Norway N-5021 5021 Bergen, Norway
Citazione:
O. Bruserud et al., "New strategies in the treatment of acute myelogenous leukemia (AML): In vitro culture of AML cells - The present use in experimental studies and the possible importance for future therapeutic approaches", STEM CELLS, 19(1), 2001, pp. 1-11

Abstract

In vitro studies of cultured native acute myelogenous leukemia (AML) blasts and cell lines have contributed significantly to our present knowledge about the pathogenesis of AML, In the present article we review different techniques for preparation and in vitro culture of AML blasts, Well-characterized serum-free in vitro conditions can now be used in experimental studies of AR IL, and this makes comparisons between different studies easier,We also describe assays for characterization of AML progenitor subsets (i.e., suspension cultures, colony assays, long-term in vitro culture, xenotransplantation in immunocompromised mice), and we discuss the possible use of AML, cell lines as experimental models in AML. Furthermore, clinical studies suggest that the in vitro growth characteristics of AML blasts assayed by short-term culture of the total native populations can be used as a predictor of prognosis after intensive chemotherapy, These in vitro assays may therefore be used for more accurate identification of prognostic parameters and thereby form a basis for the development of simplified laboratory techniques suitable for routine evaluation of patients undergoing risk-adapted therapy. However, it will be equally important to further evaluate the clinical relevance of assays for primitive ARIL progenitors, and to develop simplifiedmethods that can be used to characterize these progenitor subsets in the routine clinical evaluation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 07:57:17