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Titolo:
Decreased oxidant buffering impairs NF-kappa B activation and ICAM-1 transcription in endothelial cells
Autore:
Kefer, J; Rahman, A; Anwar, KN; Malik, AB;
Indirizzi:
Univ Illinois, Coll Med, Dept Pharmacol, Chicago, IL 60612 USA Univ Illinois Chicago IL USA 60612 Dept Pharmacol, Chicago, IL 60612 USA
Titolo Testata:
SHOCK
fascicolo: 1, volume: 15, anno: 2001,
pagine: 11 - 15
SICI:
1073-2322(200101)15:1<11:DOBINB>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
INTERCELLULAR-ADHESION MOLECULE-1; DNA-BINDING ACTIVITY; GENE-TRANSCRIPTION; REDOX REGULATION; STRAND SCISSION; ALPHA; PHOSPHORYLATION; EXPRESSION; PROTEINS; SITE;
Keywords:
TNF alpha; reactive oxygen species; glutathione; redox state; adhesion molecule; gene expression;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Rahman, A Univ Illinois, Coll Med, Dept Pharmacol, M-C868,835 S Wolcott Ave, Chicago, IL 60612 USA Univ Illinois M-C868,835 S Wolcott Ave Chicago IL USA 60612 USA
Citazione:
J. Kefer et al., "Decreased oxidant buffering impairs NF-kappa B activation and ICAM-1 transcription in endothelial cells", SHOCK, 15(1), 2001, pp. 11-15

Abstract

The DNA binding activity of the transcription factor, NF-kappaB, is regulated by the phosphorylation and degradation of its inhibitory protein, I kappaB, and post-translational modification involving redox reaction of a cysteine residue (Cys62) of NF-kappaB. We addressed the role of the redox stateof endothelial cells in modulating TNF alpha -induced NF-kappaB activity. The effects of TNF alpha on DNA-binding activity of NF-kappaB and expression of mRNA encoding ICAM-1 (an NF-kappaB-activated gene) were studied in human pulmonary artery endothelial (HPAE) cells under basal conditions and after decreasing the intracellular glutathione (GSH) concentration. HPAE cellswere treated with buthionine sulfoximine (BSO) (16 h), an inhibitor of GSHsynthesis, which caused concentration-dependent decreases in GSH concentration. Stimulation of control cells with TNF alpha resulted in reactive oxygen species (ROS) generation and activation of NF-kappaB binding to the ICAM-1 promoter and ICAM-1 transcription. However, stimulation of GSH-depleted cells with TNF alpha resulted in ROS accumulation secondary to the decreased ROS buffering capacity, and marked impairment of NF-kappaB-binding activity and ICAM-1 mRNA expression. Exposure of BSO-treated cells to the reducing agent dithiothreitol (DTT) before TNF alpha treatment or supplementation of nuclear extract (isolated after TNF alpha challenge of BSO-treated cells) with DTT significantly augmented the effect of TNF alpha on NF-kappaB-binding activity and ICAM-1 mRNA expression. Thus the oxidative modification of NF-kappaB secondary to the loss of ROS buffering capacity may regulate NF-kappaB binding to ICAM-1 promoter, and thereby ICAM-1 transcription in endothelial cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 15:20:31