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Titolo:
Neoplastic transformation and cytogenetic changes after gamma irradiation of human epithelial cells expressing telomerase
Autore:
Riches, A; Peddie, C; Rendell, S; Bryant, P; Zitzelsberger, H; Bruch, J; Smida, J; Hieber, L; Bauchinger, M;
Indirizzi:
Univ St Andrews, Sch Biol Med Sci & Human Biol, St Andrews KY16 9TS, Fife,Scotland Univ St Andrews St Andrews Fife Scotland KY16 9TS KY16 9TS, Fife,Scotland GSF Forschungszentrum Umwelt & Gesundheit, Inst Radiobiol, D-85764 Neuherberg, Germany GSF Forschungszentrum Umwelt & Gesundheit Neuherberg GermanyD-85764 any Univ Munich, Inst Radiat Biol, D-80336 Munich, Germany Univ Munich Munich Germany D-80336 Radiat Biol, D-80336 Munich, Germany
Titolo Testata:
RADIATION RESEARCH
fascicolo: 1, volume: 155, anno: 2001,
parte:, 2
pagine: 222 - 229
SICI:
0033-7587(200101)155:1<222:NTACCA>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
COMPARATIVE GENOMIC HYBRIDIZATION; RADIATION-INDUCED TRANSFORMATION; ONCOGENIC TRANSFORMATION; MALIGNANT TRANSFORMATION; IMMORTALIZED INVITRO; ALPHA-PARTICLES; HUMAN CANCER; LIFE-SPAN; IN-VITRO; TUMORS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Riches, A Univ St Andrews, Sch Biol Med Sci & Human Biol, St Andrews KY16 9TS, Fife,Scotland Univ St Andrews St Andrews Fife Scotland KY16 9TS Fife,Scotland
Citazione:
A. Riches et al., "Neoplastic transformation and cytogenetic changes after gamma irradiation of human epithelial cells expressing telomerase", RADIAT RES, 155(1), 2001, pp. 222-229

Abstract

Neoplastic transformation of human epithelial cells by radiation has previously been investigated using cell lines immortalized with viral vectors. There are disadvantages to this approach, and we report here the results of studies using a human retinal pigment epithelial cell line (340RPE-T53) immortalized by treatment with telomerase. After exposure of the cells to fractionated doses of gamma radiation, there was a marked increase in anchorage-independent growth of the surviving cells. The cloned cell lines derived from these anchorage-independent cultures exhibited an increased growth ratein vitro and were serum-independent compared with the parent cell line. The parent cell line maintained a stable diploid karyotype, The cell lines cloned after irradiation with the lower doses (10 x 2 Gy) were hypodiploid with loss of chromosome 13 and a high level amplification of 10p11.2 associated with a deletion of the remaining short arm segment of chromosome 10 distal to 10p11.2. In contrast, the cell lines cloned after irradiation with the higher doses (15 x 2 Gy) were neartetraploid with derivative chromosomes present characterized by SKY analysis. Thus this human epithelial cell lineimmortalized with telomerase provides an improved model to investigate mechanisms of radiation carcinogenesis. (C) 2001 by Radiation Research Society.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/09/20 alle ore 12:07:37