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Titolo:
In vitro models to study cellular differentiation and function in human prostate cancers
Autore:
Maitland, NJ; Macintosh, CA; Hall, J; Sharrard, M; Quinn, G; Lang, S;
Indirizzi:
Univ York, Dept Biol, YCR Canc Res Unit, York YO10 5YW, N Yorkshire, England Univ York York N Yorkshire England YO10 5YW O10 5YW, N Yorkshire, England
Titolo Testata:
RADIATION RESEARCH
fascicolo: 1, volume: 155, anno: 2001,
parte:, 2
pagine: 133 - 142
SICI:
0033-7587(200101)155:1<133:IVMTSC>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
MAMMARY EPITHELIAL-CELLS; TUMOR-SUPPRESSOR GENE; NUDE-MICE; IN-VIVO; IMMORTALIZATION; CARCINOMA; TISSUE; LINES; TRANSFORMATION; EXPRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
47
Recensione:
Indirizzi per estratti:
Indirizzo: Maitland, NJ Univ York, Dept Biol, YCR Canc Res Unit, York YO10 5YW, N Yorkshire, England Univ York York N Yorkshire England YO10 5YW rkshire, England
Citazione:
N.J. Maitland et al., "In vitro models to study cellular differentiation and function in human prostate cancers", RADIAT RES, 155(1), 2001, pp. 133-142

Abstract

To augment the currently available models of human prostate cancer in vitro, we have established extended life-span epithelial cultures from biopsiesof well-differentiated prostate cancers. The genetic identity of the target cells was assessed by allelotyping, using microsatellites located on chromosome 8p, and microdissection of tissues and primary cell cultures. Cells with an extended life span (PxE6) were derived by recombinant retrovirus infection to introduce the human papilloma virus E6 gene (epithelial cells). Immunophenotyping of the resultant cell strains confirmed retention of differentiated cell functions, and the genotype of the E6-expressing epithelialcells was stable, while SV40-immortalized cultures were more unstable, leading to tetraploidy. All PxE6 cells eventually senesced, but an immortalized epithelial culture, P4E6, was derived from one of the epithelial cultures. The properties of this cell line, which remains close to diploid, are similar to those of early prostate cancer cells, and it retains expression of many prostate-associated antigens, such as prostate-specific antigen (PSA). (C) 2001 by Radiation Research Society.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/03/20 alle ore 23:04:01