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Titolo:
Analysis of habituated embryogenic lines in Asparagus officinalis L.: growth characteristics, hormone content and ploidy level of calli and regenerated plants
Autore:
Limanton-Grevet, A; Sotta, B; Brown, S; Jullien, M;
Indirizzi:
INRA Versailles, INA PG Biol Semences, UMR INRA, F-78026 Versailles, France INRA Versailles Versailles France F-78026 RA, F-78026 Versailles, France J Murionnet GFA, Lab Invitro, F-41230 Soings En Sologne, France J Murionnet GFA Soings En Sologne France F-41230 ings En Sologne, France Asparagus BV, NL-5960 AE Horst, Netherlands Asparagus BV Horst Netherlands NL-5960 AE NL-5960 AE Horst, Netherlands Univ Paris 06, Lab Physiol Dev Plantes, UMR Physiol Cellulaire & Mol Plantes, F-75252 Paris 05, France Univ Paris 06 Paris France 05 re & Mol Plantes, F-75252 Paris 05, France CNRS, Inst Sci Vegetales, F-91198 Gif Sur Yvette, France CNRS Gif Sur Yvette France F-91198 tales, F-91198 Gif Sur Yvette, France
Titolo Testata:
PLANT SCIENCE
fascicolo: 1, volume: 160, anno: 2000,
pagine: 15 - 26
SICI:
0168-9452(200012)160:1<15:AOHELI>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
DIRECT SOMATIC EMBRYOGENESIS; GENOTYPE; CULTURE; PROTOPLASTS; MAIZE;
Keywords:
Asparagus officinalis L.; somatic embryogenesis; habituation; mutation; hormone; flow cytometry; ploidy level;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Jullien, M INRA Versailles, INA PG Biol Semences, UMR INRA, Route St Cyr, F-78026 Versailles, France INRA Versailles Route St Cyr Versailles France F-78026 France
Citazione:
A. Limanton-Grevet et al., "Analysis of habituated embryogenic lines in Asparagus officinalis L.: growth characteristics, hormone content and ploidy level of calli and regenerated plants", PLANT SCI, 160(1), 2000, pp. 15-26

Abstract

Habituated asparagus embryogenic lines derived from eleven genotypes were maintained on hormone-free medium and grew actively through secondary embryogenesis. Secondary embryos were of single cell origin and emerged from thetransversal division of some epidermal or subepidermal cotyledonary cells of primary embryos. The intensity of secondary embryogenesis was found to be variable between embryogenic lines. Plants regenerated from three of these lines have been previously demonstrated to carry a mutation whose phenotype was the direct appearance of somatic embryos on apices or nodes culturedon hormone-free medium. Habituated lines of embryogenic calli and various tissues of embryogenic mutant and wild type plants were analysed for their hormonal content in ABA, IAA, iP, Z and their metabolites ABA-GE, iPA, iMP,ZR. No significant difference was found between different embryogenic lines, except the level of iPA, or between cladophyll or apex cultures of mutant and wild type plants. Flow cytometry analyses indicated only 34% of the embryogenic lines were diploid, most of the others bring tetraploid, but 62%of regenerated plants from these lines were diploid. This indicated the process of maturation and conversion selected diploid embryos ill the embryogenic lines. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 19:46:00