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Titolo:
Entamoeba histolytica: production of nitric oxide and in situ activity of NADPH diaphorase in amebic liver abscess of hamsters
Autore:
Pacheco-Yepez, J; Campos-Rodriguez, R; Shibayama, M; Ventura-Juarez, J; Serrano-Luna, J; Tsutsumi, V;
Indirizzi:
IPN, CINVESTAV, Dept Patol Expt, Mexico City 07360, DF, Mexico IPN MexicoCity DF Mexico 07360 Patol Expt, Mexico City 07360, DF, Mexico Natl Polytechn Inst, Sch Med, Dept Biochem, Mexico City, DF, Mexico Natl Polytechn Inst Mexico City DF Mexico ochem, Mexico City, DF, Mexico Natl Polytechn Inst, Interdisciplinary Ctr Hlth Sci, Mexico City, DF, Mexico Natl Polytechn Inst Mexico City DF Mexico h Sci, Mexico City, DF, Mexico La Salle Univ, Sch Med, Electron Microscopy Unit, Mexico City, DF, Mexico La Salle Univ Mexico City DF Mexico oscopy Unit, Mexico City, DF, Mexico
Titolo Testata:
PARASITOLOGY RESEARCH
fascicolo: 1, volume: 87, anno: 2001,
pagine: 49 - 56
SICI:
0932-0113(200101)87:1<49:EHPONO>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
L-ARGININE; SYNTHASE IMMUNOREACTIVITY; GLUCOCORTICOIDS INHIBIT; TOXOPLASMA-GONDII; IN-VIVO; MACROPHAGE CYTOTOXICITY; NITRATE BIOSYNTHESIS; MURINE LEISHMANIASIS; TRYPANOSOMA-CRUZI; REACTIVE NITROGEN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
66
Recensione:
Indirizzi per estratti:
Indirizzo: Tsutsumi, V IPN, CINVESTAV, Dept Patol Expt, Av IPN 2508, Mexico City 07360, DF, Mexico IPN Av IPN 2508 Mexico City DF Mexico 07360 07360, DF, Mexico
Citazione:
J. Pacheco-Yepez et al., "Entamoeba histolytica: production of nitric oxide and in situ activity of NADPH diaphorase in amebic liver abscess of hamsters", PARASIT RES, 87(1), 2001, pp. 49-56

Abstract

Entamoeba histolytica trophozoites were inoculated into the liver of hamsters and serum nitrate/nitrite levels [expressed as nitric oxide (NO) production] were determined at different times during amebic liver abscess (ALA) development. We also tested the effects of NO synthase (NOS) inhibitors such as NG-nitro-L-arginine methyl ester (L-NAME), aminoguanidine, and dexamethasone during ALA production. Since NOS activity has been correlated with expression of reduced nicotinamide adenine dinucleotide phosphate diaphorase(NADPHd) in tissues, we performed histochemistry studies to determine the activity of the latter in livers infected with E. histolytica trophozoites. Production of NO in serum was directly proportional to the size of ALAs, and NOS inhibitors caused low levels of NO and smaller ALAs. Our data suggest that NO does not have any lytic effect on E. histolytica trophozoites andis, therefore incapable of providing protection against the amebic liver infection. In addition, NADPHd activity was detected histochemically in hepatocytes and inflammatory cells associated with focal necrosis containing trophozoites. The positive reactivity observed in these parasites may be attributable to a close biochemical similarity of NADPHd to the NADPH:flavin oxidoreductase described in E. histolytica by other investigators.

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Documento generato il 14/08/20 alle ore 15:37:49