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Titolo:
Determination of S-nitrosohemoglobin using a solid-state amperometric sensor
Autore:
Palmerini, CA; Arienti, G; Palombari, R;
Indirizzi:
Univ Perugia, Dipartimento Biol Cellulare & Mol, I-06127 Perugia, Italy Univ Perugia Perugia Italy I-06127 llulare & Mol, I-06127 Perugia, Italy Univ Perugia, Ist Biochim & Chim Med, I-06127 Perugia, Italy Univ PerugiaPerugia Italy I-06127 im & Chim Med, I-06127 Perugia, Italy Univ Perugia, Dipartimento Chim, I-06125 Perugia, Italy Univ Perugia Perugia Italy I-06125 rtimento Chim, I-06125 Perugia, Italy
Titolo Testata:
NITRIC OXIDE-BIOLOGY AND CHEMISTRY
fascicolo: 6, volume: 4, anno: 2000,
pagine: 546 - 549
SICI:
1089-8603(200012)4:6<546:DOSUAS>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
NITRIC-OXIDE; OXYGEN-BINDING; HEMOGLOBIN; NITROSOTHIOLS; NITROSOGLUTATHIONE; NITROSATION; MECHANISM;
Keywords:
hemoglobin; S-nitrosohemoglobin; nitrosothiols; nitric oxide (NO);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
22
Recensione:
Indirizzi per estratti:
Indirizzo: Palmerini, CA Univ Perugia, Dipartimento Biol Cellulare & Mol, Via Giochetto, I-06127 Perugia, Italy Univ Perugia Via Giochetto Perugia Italy I-06127 gia, Italy
Citazione:
C.A. Palmerini et al., "Determination of S-nitrosohemoglobin using a solid-state amperometric sensor", NITRIC OXID, 4(6), 2000, pp. 546-549

Abstract

Nitric oxide (NO, nitrogen monoxide), generated in biological systems, plays important roles as a regulatory molecule. Its ability to bind to hemoglobin (Hb) iron is well known. Moreover, it may lose an electron, forming thenitrosonium ion, involved in the synthesis of nitrosothiols (RSNO). It hasbeen suggested that S-nitrosohemoglobin (SNO-Hb) may act as a reservoir ofNO. The S-nitrosylation of Hb can be detected after the incubation of CysNO and Hb for 60 mim with a molecular ratio (CysNO/hem) of 1:1. Upon increasing the ratio to 10:1, about 50% of total Hb (100% of beta -chain -SH 93) was derivatized in 60 min. In this paper, we describe a new method for the quantitative assay of SNO-Hb, after the liberation of NO by Cu2+/Cu+ and thesimultaneous assessment of NO by solid-state amperometric sensor. The assay described by us is sensitive, rapid, easy to perform, and inexpensive. For this reason, we believe that it may represent an important analytical improvement for the study of the S-transnitrosylation reactions between RSNO and the Hb Cys-beta 93 and SNO-Hb and glutathione. (C) 2000 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 13/07/20 alle ore 11:07:34