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Titolo:
Modulation of alpha 2 beta 4 neuronal nicotinic acetylcholine receptors byzinc
Autore:
Garcia-Colunga, L; Gonzalez-Herrera, M; Miledi, R;
Indirizzi:
Univ Nacl Autonoma Mexico, Ctr Neurobiol, Juriquilla 76001, Queretaro, Mexico Univ Nacl Autonoma Mexico Juriquilla Queretaro Mexico 76001 etaro, Mexico Univ Calif Irvine, Dept Neurobiol & Behav, Cellular & Mol Neurobiol Lab, Irvine, CA 92697 USA Univ Calif Irvine Irvine CA USA 92697 Neurobiol Lab, Irvine, CA 92697 USA
Titolo Testata:
NEUROREPORT
fascicolo: 1, volume: 12, anno: 2001,
pagine: 147 - 150
SICI:
0959-4965(20010122)12:1<147:MOA2B4>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
HIPPOCAMPAL-NEURONS; BLOCKAGE; ALPHA(7); AGONIST; MUSCLE; IONS; 5-HYDROXYTRYPTAMINE; PERMEABILITY; INHIBITION; LANTHANUM;
Keywords:
neuronal receptors; receptor potentiation; Xenopus oocytes; zinc modulation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Miledi, R Univ Nacl Autonoma Mexico, Ctr Neurobiol, Campus Juriquilla,Apartado Postal 1-1141, Juriquilla 76001, Queretaro, Mexico Univ Nacl Autonoma Mexico Campus Juriquilla,Apartado Postal 1-1141 Juriquilla Queretaro Mexico76001
Citazione:
L. Garcia-Colunga et al., "Modulation of alpha 2 beta 4 neuronal nicotinic acetylcholine receptors byzinc", NEUROREPORT, 12(1), 2001, pp. 147-150

Abstract

A study was made of the modulation of nicotinic acetylcholine receptors bythe divalent cation zinc. Rat neuronal nicotinic receptors (alpha2 beta4) were expressed in Xenopus oocytes and membrane currents evoked by acetylcholine (ACh currents) were recorded using a two microelectrode voltage clamp. In non-injected oocytes, or in oocytes expressing alpha2 beta4 receptors, Zn2+ by itself (1 muM-4 mM) generated only very small membrane currents. Incontrast, in oocytes expressing alpha2 beta4 receptors, Zn2+ greatly and reversibly increased the ACh current, without affecting considerably its time course. The ACh current potentiation by Zn2+ was weakly dependent on the membrane potential (2.33 +/- 0.10 times the control current at -100 mV vs 2.04 +/- 0.06 at -60 mV, suggesting that Zn2+ interacts with the receptor inthe vestibule of the ion channel or at an external domain of the protein. The inward rectification of control and Zn2+-potentiated ACh-currents was similar. We conclude that Zn2+ positively and reversibly modulates neuronal nicotinic receptors in a practically voltage-independent manner and withoutaffecting their rate of desensitization. These results will help to understand better the roles played by Zn2+ in brain functions. NeuroReport 12:147-150 (C) 2001 Lippincott Williams & Wilkins.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/01/20 alle ore 10:10:36