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Titolo:
Targeted disruption of luteinizing hormone/human chorionic gonadotropin receptor gene
Autore:
Lei, ZM; Mishra, S; Zou, W; Xu, B; Foltz, M; Li, X; Rao, CV;
Indirizzi:
Univ Louisville, Hlth Sci Ctr, Dept Obstet & Gynecol, Div Basic Sci Res, Louisville, KY 40292 USA Univ Louisville Louisville KY USA 40292 Sci Res, Louisville, KY 40292 USA
Titolo Testata:
MOLECULAR ENDOCRINOLOGY
fascicolo: 1, volume: 15, anno: 2001,
pagine: 184 - 200
SICI:
0888-8809(200101)15:1<184:TDOLHC>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
FOLLICLE-STIMULATING-HORMONE; GERM-CELL APOPTOSIS; HYPOTHALAMIC GT1-7 NEURONS; MICE LACKING; INACTIVATING MUTATIONS; DEFICIENT MICE; LH RECEPTORS; TESTIS; PROLIFERATION; MATURATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
79
Recensione:
Indirizzi per estratti:
Indirizzo: Rao, CV Univ Louisville, Hlth Sci Ctr, Dept Obstet Gynecol, 438 MDR Bldg, Louisville, KY 40292 USA Univ Louisville 438 MDR Bldg Louisville KY USA 40292 KY 40292 USA
Citazione:
Z.M. Lei et al., "Targeted disruption of luteinizing hormone/human chorionic gonadotropin receptor gene", MOL ENDOCR, 15(1), 2001, pp. 184-200

Abstract

LH/hCG receptors were disrupted by gene targeting in embryonic stem cells,The disruption resulted in infertility in both sexes. The gonads containedno receptor mRNA or receptor protein. Serum LH levels were greatly elevated, and FSH levels were moderately elevated in both sexes; estradiol and progesterone levels decreased but were not totally suppressed in females; testosterone levels were dramatically decreased and estradiol levels moderatelyelevated in males. The external and internal genitalia were grossly underdeveloped in both sexes. Abnormalities included ambiguous vaginal opening, abdominal testes, micropenis, dramatically decreased weights of the gonads and reproductive tract, arrested follicular growth beyond antral stage, disarray of seminiferous tubules, diminished number and hypotrophy of Leydig cells, and spermatogenic arrest beyond the round spermatid stage. LH/hCG receptor gene disruption had no effect on FSH receptor mRNA levels in ovaries and testes, progesterone receptor (PR) levels in ovaries and androgen receptor (AR) levels in testes. However, it caused a dramatic decrease in SMR andestrogen receptor-alpha (ER alpha) mRNA levels and an increase in ER beta mRNA levels in both ovaries and testes. Estradiol and progesterone replacement therapy in females and testosterone replacement in males, to determine whether phenotype and biochemical changes were a consequence of decreased gonadal steroid levels or due to a loss of LH signaling, revealed complete restoration of some and partial restoration of others. Nevertheless, the animals remained infertile. It is anticipated that the LH receptor knockout animals will increase our current understanding of gonadal and nongonadal actions of LH and hCG.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/03/20 alle ore 23:11:27