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Titolo:
Multiple roles of Arf1 GTPase in the yeast exocytic and endocytic pathways
Autore:
Yahara, N; Ueda, T; Sato, K; Nakano, A;
Indirizzi:
RIKEN, Mol Membrane Biol Lab, Wako, Saitama 3510198, Japan RIKEN Wako Saitama Japan 3510198 e Biol Lab, Wako, Saitama 3510198, Japan
Titolo Testata:
MOLECULAR BIOLOGY OF THE CELL
fascicolo: 1, volume: 12, anno: 2001,
pagine: 221 - 238
SICI:
1059-1524(200101)12:1<221:MROAGI>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
ADP-RIBOSYLATION FACTOR; AP-3 ADAPTER COMPLEX; SACCHAROMYCES-CEREVISIAE; ENDOPLASMIC-RETICULUM; SECRETORY PATHWAY; PROTEIN-TRANSPORT; GOLGI MEMBRANES; BINDING PROTEIN; PHOSPHOLIPASE-D; SEC7 DOMAIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
83
Recensione:
Indirizzi per estratti:
Indirizzo: Nakano, A RIKEN, Mol Membrane Biol Lab, 2-1 Wako, Wako, Saitama 3510198, Japan RIKEN 2-1 Wako Wako Saitama Japan 3510198 Saitama 3510198, Japan
Citazione:
N. Yahara et al., "Multiple roles of Arf1 GTPase in the yeast exocytic and endocytic pathways", MOL BIOL CE, 12(1), 2001, pp. 221-238

Abstract

ADP-ribosylation factors, a family of small GTPases, are believed to be key regulators of intracellular membrane traffic. However, many biochemical in vitro experiments have led to different models for their involvement in various steps of vesicular transport, and their precise role in living cellsis still unclear. We have taken advantage of the powerful yeast genetic system and screened for temperature-sensitive (ts) mutants of the ARF1 gene from Saccharomyces cerevisiae. By random mutagenesis of the whole open reading frame of ARF1 by error-prone PCR, we isolated eight mutants and examinedtheir phenotypes. arf1 ts mutants showed a variety of transport defects and morphological alterations in an allele-specific manner. Furthermore, intragenic complementation was observed between certain pairs of mutant alleles, both for cell growth and intracellular transport. These results demonstrate that the single Arf1 protein is indeed involved in many different steps of intracellular transport in vivo and that its multiple roles may be dissected by the mutant alleles we constructed.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/10/20 alle ore 08:55:31