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Titolo:
Mitochondrial Ca2+-induced Ca2+ release mediated by the Ca2+ uniporter
Autore:
Montero, M; Alonso, MT; Albillos, A; Garcia-Sancho, J; Alvarez, J;
Indirizzi:
Univ Valladolid, Inst Mol Biol & Genet, E-47005 Valladolid, Spain Univ Valladolid Valladolid Spain E-47005 enet, E-47005 Valladolid, Spain Fac Med, Dept Bioquim & Biol Mol & Fisiol, CSIC, E-47005 Valladolid, SpainFac Med Valladolid Spain E-47005 Fisiol, CSIC, E-47005 Valladolid, Spain Univ Autonoma Madrid, Fac Med, Dept Farmacol & Terapeut, Inst Farmacol Teofilo Hernando, E-28029 Madrid, Spain Univ Autonoma Madrid Madrid Spain E-28029 ernando, E-28029 Madrid, Spain
Titolo Testata:
MOLECULAR BIOLOGY OF THE CELL
fascicolo: 1, volume: 12, anno: 2001,
pagine: 63 - 71
SICI:
1059-1524(200101)12:1<63:MCCRMB>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
ADRENAL CHROMAFFIN CELLS; RAT-LIVER MITOCHONDRIA; PERMEABILITY TRANSITION PORE; ENDOPLASMIC-RETICULUM; SYMPATHETIC NEURONS; HEART-MITOCHONDRIA; TARGETED AEQUORIN; EFFLUX MECHANISM; CALCIUM SIGNALS; INTACT-CELLS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
51
Recensione:
Indirizzi per estratti:
Indirizzo: Alvarez, J Univ Valladolid, Inst Mol Biol & Genet, E-47005 Valladolid, Spain Univ Valladolid Valladolid Spain E-47005 05 Valladolid, Spain
Citazione:
M. Montero et al., "Mitochondrial Ca2+-induced Ca2+ release mediated by the Ca2+ uniporter", MOL BIOL CE, 12(1), 2001, pp. 63-71

Abstract

We have reported that a population of chromaffin cell mitochondria takes up large amounts of Ca2+ during cell stimulation. The present study focuses on the pathways for mitochondrial Ca2+ efflux. Treatment with protonophoresbefore cell stimulation abolished mitochondrial Ca2+ uptake and increased the cytosolic [Ca2+] ([Ca2+](c)) peak induced by the stimulus. Instead, when protonophores were added after cell stimulation, they did not modify [Ca2](c) kinetics and inhibited Ca2+ release from Ca2+-loaded mitochondria. This effect was due to inhibition of mitochondrial Na+/Ca2+ exchange, becauseblocking this system with CGP37157 produced no further effect. Increasing extramitochondrial [Ca2+](c) triggered fast Ca2+ release from these depolarized Ca2+-loaded mitochondria, both in intact or permeabilized cells. Theseeffects of protonophores were mimicked by valinomycin, but not by nigericin. The observed mitochondrial Ca2+- induced Ca2+ release response was insensitive to cyclosporin A and CGP37157 but fully blocked by ruthenium red, suggesting that it may be mediated by reversal of the Ca2+ uniporter. This novel kind of mitochondrial Ca2+-induced Ca2+ release might contribute to Ca2 clearance from mitochondria that become depolarized during Ca2+ overload.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/04/20 alle ore 10:28:50