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Titolo:
Computer-generated, three-dimensional reconstruction of histological parallel serial sections displaying microvascular and glandular structures in human endometrium
Autore:
Manconi, F; Markham, R; Cox, G; Kable, E; Fraser, IS;
Indirizzi:
Univ Sydney, Queen Elizabeth II Res Inst Mothers & Infants, Dept Obstet & Gynaecol, Sydney, NSW 2006, Australia Univ Sydney Sydney NSW Australia 2006 naecol, Sydney, NSW 2006, Australia Univ Sydney, Australian Key Ctr Microscopy & Microanal, Sydney, NSW 2006, Australia Univ Sydney Sydney NSW Australia 2006 roanal, Sydney, NSW 2006, Australia Univ Sydney, Electron Microscope Unit, Sydney, NSW 2006, Australia Univ Sydney Sydney NSW Australia 2006 e Unit, Sydney, NSW 2006, Australia
Titolo Testata:
MICRON
fascicolo: 4, volume: 32, anno: 2001,
pagine: 449 - 453
SICI:
0968-4328(200106)32:4<449:CTROHP>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
3-DIMENSIONAL RECONSTRUCTION; PERITONEAL ENDOMETRIOSIS; VISUALIZATION; DISTORTION; TISSUE;
Keywords:
3D computer reconstruction; microvascular; endometrium; kontron; VoxBlast;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
19
Recensione:
Indirizzi per estratti:
Indirizzo: Manconi, F Univ Sydney, Queen Elizabeth II Res Inst Mothers & Infants, Dept Obstet & Gynaecol, Sydney, NSW 2006, Australia Univ Sydney Sydney NSW Australia 2006 ney, NSW 2006, Australia
Citazione:
F. Manconi et al., "Computer-generated, three-dimensional reconstruction of histological parallel serial sections displaying microvascular and glandular structures in human endometrium", MICRON, 32(4), 2001, pp. 449-453

Abstract

This paper describes a technique to develop high-resolution three-dimensional (3D) images of microvasculature structures in curettage, hysterectomy or endometrial resection biopsies using parallel histological serial sections. Employing a labelled streptavidin-biotin-alkaline phosphatase (LSAB(+)) method and visualising by using DAB(+) with the primary antibody, mouse anti human Q-Bend-10, the images were directly digitised from a light microscope into the KS400 Universal Image Processing and Analysis software via a CCD colour camera; binary images of the structures were created and the binary images were exported into VoxBlast 3D rendering software to view still and rotating 3D images on a computer monitor. This in turn enabled hard copies of the full sequence to be printed. (C) 2001 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/08/20 alle ore 12:13:42