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Titolo:
Cloning and characterization of the gene encoding periplasmic 2 ',3 '-cyclic phosphodiesterase of Yersinia enterocolitica O : 8
Autore:
Trulzsch, K; Roggenkamp, A; Pelludat, C; Rakin, A; Jacobi, CA; Heesemann, J;
Indirizzi:
Univ Munich, Max von Pettenkofer Inst Med Mikrobiol & Hyg, D-80336 Munich,Germany Univ Munich Munich Germany D-80336 krobiol & Hyg, D-80336 Munich,Germany
Titolo Testata:
MICROBIOLOGY-UK
, volume: 147, anno: 2001,
parte:, 1
pagine: 203 - 213
SICI:
1350-0872(200101)147:<203:CACOTG>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
ESCHERICHIA-COLI; SALMONELLA-TYPHIMURIUM; MESSENGER-RNA; REP SEQUENCES; EXPRESSION; VIRULENCE; 5'-NUCLEOTIDASE; CONSTRUCTION; PATHOGENESIS; PLASMIDS;
Keywords:
cpdB gene; 2 ',3 '-cAMP; ERIC; cAMP-CRP-binding site;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
47
Recensione:
Indirizzi per estratti:
Indirizzo: Heesemann, J Univ Munich, Max von Pettenkofer Inst Med Mikrobiol & Hyg, Pettenkoferstr 9A, D-80336 Munich, Germany Univ Munich Pettenkoferstr 9A Munich Germany D-80336 Germany
Citazione:
K. Trulzsch et al., "Cloning and characterization of the gene encoding periplasmic 2 ',3 '-cyclic phosphodiesterase of Yersinia enterocolitica O : 8", MICROBIO-UK, 147, 2001, pp. 203-213

Abstract

The gene encoding periplasmic 2',3'-cyclic phosphodiesterase in Yersinia enterocolitica O:8 (designated cpdB), was cloned and expressed in Escherichia coli. This enzyme enables Y. enterocolitica to grow on 2',3'-cAMP as a sole source of carbon and energy. Sequencing and analysis of a 3 kb EcoRI fragment containing the cpdB gene revealed an open reading frame of 1179 bp, corresponding to a protein with a molecular mass of 71 kDa. The first 25 amino acid residues show features of a typical prokaryotic signal sequence. The predicted molecular mass of the mature peptide is therefore in agreement with the molecular mass estimated by SDS gel electrophoresis (68 kDa). The putative cpdB promoter region contains two possible -10 and -35 regions. Furthermore, the 5' untranslated region contains sequences with significant homology to the cyclic AMP-cyclic AMP receptor protein binding site and the sigma (28) consensus. This region is interrupted by an enterobacterial repetitive intergenic consensus (ERIC) sequence. Deletion of the ERIC element from the cpdB promoter region had no effect on cpdB expression. In the 3' untranslated region, a possible rho-independent transcriptional terminator was identified. The deduced amino acid sequence of the Y. enterocolitica CpdBprotein shows 76% identity with CpdB of Salmonella typhimurium and E. coli. CpdB of Y. enterocolitica is exported to the periplasmic space. An isogenic Y. enterocolitica cpdB mutant strain, constructed by allelic exchange, was no longer able to grow on 2',3'-cAMP as sole source of carbon and energy. The CpdB mutant showed no significant change in virulence in an oral and intravenous mouse infection model.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/01/20 alle ore 06:50:41