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Titolo:
PCR detection of Capnocytophaga species in dental plaque samples from children aged 2 to 12 years
Autore:
Hayashi, F; Okada, M; Zhong, XB; Miura, K;
Indirizzi:
Hiroshima Univ, Fac Dent, Dept Pediat Dent, Minami Ku, Hiroshima 7348553, Japan Hiroshima Univ Hiroshima Japan 7348553 nami Ku, Hiroshima 7348553, Japan
Titolo Testata:
MICROBIOLOGY AND IMMUNOLOGY
fascicolo: 1, volume: 45, anno: 2001,
pagine: 17 - 22
SICI:
0385-5600(2001)45:1<17:PDOCSI>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
NEGATIVE GLIDING BACTERIA; POLYMERASE CHAIN-REACTION; PERIODONTALLY HEALTHY-INDIVIDUALS; ACTINOBACILLUS-ACTINOMYCETEMCOMITANS; PORPHYROMONAS-GINGIVALIS; PREPUBERTAL PERIODONTITIS; BACTEROIDES-FORSYTHUS; ADULT PERIODONTITIS; MICROORGANISMS; IDENTIFICATION;
Keywords:
PCR; dental plaque; Capnocytophaga; children;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Okada, M Hiroshima Univ, Fac Dent, Dept Pediat Dent, Minami Ku, 1-2-3 Kasumi, Hiroshima 7348553, Japan Hiroshima Univ 1-2-3 Kasumi Hiroshima Japan 7348553 48553, Japan
Citazione:
F. Hayashi et al., "PCR detection of Capnocytophaga species in dental plaque samples from children aged 2 to 12 years", MICROB IMMU, 45(1), 2001, pp. 17-22

Abstract

The purpose of this study was to detect the presence of Capnocytophaga sputigena, C, ochracea, and C. gingivalis in plaque samples from the toothbrushes of 122 children, using a polymerase chain reaction (PCR) method. The subjects were 25, 85, and 12 children with healthy gingiva, gingivitis, and periodontitis, respectively, ranging in age from 2-12 years old. Plaque samples were collected from all erupted tooth sites using a sterile toothbrush. The mean amount of DNA recovered from the samples was approximately 193 mug, which was deemed sufficient for performing a PCR-based survey. C, sputigena prevalence in healthy, gingivitis, and periodontitis subjects was 48.0%, 36.5% and 25.0%, respectively, that for C. ochracea was 100%, 89.4%, and 50.0%, respectively, and that for C, gingivalis was 96.0%, 84.7%, and 75.0%, respectively. The lowest age of positive subjects was approximately 2 years. Our results showed that C. sputigena was moderately prevalent, whereas C, ochracea and C, gingivalis were commonly detected in the oral cavities of the tested children, suggesting that all of these species become established in the early years.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/09/20 alle ore 07:05:05