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Titolo:
17 beta-estradiol inhibits proliferation of cultured vascular smooth muscle cells induced by lysophosphatidylcholine via a nongenomic antioxidant mechanism
Autore:
Yoon, BK; Oh, WJ; Kessel, B; Roh, CR; Choi, D; Lee, JH; Kim, DK;
Indirizzi:
Sung Kyun Kwan Univ, Sch Med, Samsung Med Ctr, Dept Med, Seoul 135710, South Korea Sung Kyun Kwan Univ Seoul South Korea 135710 , Seoul 135710, South Korea Sung Kyun Kwan Univ, Sch Med, Samsung Med Ctr, Dept Obstet & Gynecol, Seoul 135710, South Korea Sung Kyun Kwan Univ Seoul South Korea 135710 , Seoul 135710, South Korea Univ Hawaii, Dept Obstet & Gynecol, Honolulu, HI 96822 USA Univ Hawaii Honolulu HI USA 96822 bstet & Gynecol, Honolulu, HI 96822 USA
Titolo Testata:
MENOPAUSE-THE JOURNAL OF THE NORTH AMERICAN MENOPAUSE SOCIETY
fascicolo: 1, volume: 8, anno: 2001,
pagine: 58 - 64
SICI:
1072-3714(200101/02)8:1<58:1BIPOC>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
LOW-DENSITY-LIPOPROTEIN; ESTROGEN-RECEPTOR-BETA; ER-BETA; HEART-DISEASE; ESTRADIOL; ALPHA; EXPRESSION; CLONING; ARTERY; INJURY;
Keywords:
estrogen; atherosclerosis; antioxidant; smooth muscle cell; lysophosphatidylcholine;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Kim, DK Sung Kyun Kwan Univ, Sch Med, Samsung Med Ctr, Dept Med, 50 Ilwon Dong, Seoul 135710, South Korea Sung Kyun Kwan Univ 50 Ilwon Dong Seoul South Korea 135710 Korea
Citazione:
B.K. Yoon et al., "17 beta-estradiol inhibits proliferation of cultured vascular smooth muscle cells induced by lysophosphatidylcholine via a nongenomic antioxidant mechanism", MENOPAUSE, 8(1), 2001, pp. 58-64

Abstract

Objective: Lysophosphatidylcholine (lysoPC), an active component of oxidized low-density lipoprotein, stimulates proliferation of vascular smooth muscle cells (VSMC), We investigated the direct impact of 17 beta -estradiol (E-2) on the proliferation of VSMC from rat aorta. Results: VSMC derived from both female and male rats expressed estrogen receptors alpha and beta, Treatments with 1% fetal bovine serum or 5 muM lysoPC increased the incorporation of [H-3]thymidine in VSMC obtained from female rats. 17 beta -E-2 did not alter the response to fetal bovine serum, butsignificantly suppressed the enhanced deoxyribonucleic acid synthesis which had been induced by lysoPC in a dose-dependent manner(10(-4)-10(-6) M). Estrogen also inhibited the proliferation of VSMC from male animals. ICI 182,780, a specific estrogen receptor antagonist, and 17 alpha -E-2, an inactive form of estradiol, also decreased the mitogenic response to lysoPC in VSMC, In addition, N-acetyl-L-cysteine, a potent antioxidant, inhibited the lysoPC effect. Flow cytometric analysis using the oxidation-sensitive probe 2',7'-dichlorofluorescin diacetate revealed that elevated intracellular formation of reactive oxygen species elicited with lysoPC was depressed significantly by 17 beta -E-2, ICI 182,780, or 17 alpha -E-2 as well as by N-acetyl-L-cysteine. Conclusion: 17 beta -E-2 inhibits in vitro VSMC proliferation induced by lysoPC via a nongenomic antioxidant mechanism.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/09/20 alle ore 00:59:53