Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Characterization of constitutive and putative differentially expressed mRNAs by means of expressed sequence tags, differential display reverse transcriptase-PCR and randomly amplified polymorphic DNA-PCR from the sand fly vector Lutzomyia longipalpis
Autore:
Ramalho-Ortigao, JM; Temporal, P; de Oliveira, SMP; Barbosa, AF; Vilela, ML; Rangel, EF; Brazil, RP; Traub-Cseko, YM;
Indirizzi:
Inst Oswaldo Cruz, Dept Bioquim & Biol Mol, BR-21045900 Rio De Janeiro, Brazil Inst Oswaldo Cruz Rio De Janeiro Brazil BR-21045900 BCDe Janeiro, Brazil Inst Oswaldo Cruz, Dept Entomol, BR-21045900 Rio De Janeiro, Brazil Inst Oswaldo Cruz Rio De Janeiro Brazil BR-21045900 BCDe Janeiro, Brazil FIOCRUZ, Ctr Pesquisas Rene Rachou, Belo Horizonte, MG, Brazil FIOCRUZ Belo Horizonte MG Brazil Rene Rachou, Belo Horizonte, MG, Brazil
Titolo Testata:
MEMORIAS DO INSTITUTO OSWALDO CRUZ
fascicolo: 1, volume: 96, anno: 2001,
pagine: 105 - 111
SICI:
0074-0276(200101)96:1<105:COCAPD>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
MOSQUITO ANOPHELES-GAMBIAE; REPAIR GENE RAD6; V-ATPASE; LEISHMANIA-DONOVANI; BLOOD MEAL; LIPOPHOSPHOGLYCAN; GUT; DROSOPHILA; MIDGUT; MAXADILAN;
Keywords:
sand fly; Lutzomyia longipalpis; Leishmania; differential; display; expressed sequence tags;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Traub-Cseko, YM Inst Oswaldo Cruz, Dept Bioquim & Biol Mol, Av Brasil 4365, BR-21045900 Rio De Janeiro, Brazil Inst Oswaldo Cruz Av Brasil 4365 Rio De Janeiro Brazil BR-21045900 BC
Citazione:
J.M. Ramalho-Ortigao et al., "Characterization of constitutive and putative differentially expressed mRNAs by means of expressed sequence tags, differential display reverse transcriptase-PCR and randomly amplified polymorphic DNA-PCR from the sand fly vector Lutzomyia longipalpis", MEM I OSW C, 96(1), 2001, pp. 105-111

Abstract

Molecular studies of insect disease vectors are of paramount importance for understanding parasite-vector relationship. Advances in this area led to important findings regarding changes in vectors' physiology upon blood feeding and parasite infection. Mechanisms for interfering with the vectorial capacity of insects responsible for the transmission of diseases such as malaria, Chagas disease and dengue fever are being devised with the ultimate goal of developing transgenic insects. A primary necessity for this goal is information on gene expression and control in the target insect. Our group is investigating molecular aspects of the interaction between Leishmania parasites and Lutzomyia sand flies. As an initial step in our studies we haveused random sequencing of cDNA clones from two expression libraries made from head/thorax and abdomen of sugar fed L. longipalpis for the identification of expressed sequence tags (EST). We applied differential display reverse transcriptase-PCR and randomly amplified polymorphic DNA-PCR to characterize differentially expressed mRNA from sugar and blood fed insects, and, in one case, from a L. (V.) braziliensis-infected L. longipalpis. We identified 37 cDNAs that have shown homology to known sequences from GeneBank. Of these, 32 cDNAs code for constitutive proteins such as zinc finger protein,glutamine synthetase, G binding protein, ubiquitin conjugating enzyme. Three are putative differentially expressed cDNAs from blood fed and Leishmania-infected midgut, a chitinase, a V-ATPase and a MAP kinase. Finally, two sequences are homologous to Drosophila melanogaster gene products recently discovered through the Drosophila genome initiative.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 09:20:39