Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Dietary cholesterol induces changes in molecular species of hepatic microsomal phosphatidylcholine
Autore:
Bernasconi, AM; Garda, HA; Brenner, RR;
Indirizzi:
Fac Ciencias, UNLP, CONICET, Inst Invest Bioquim La Plata, RA-1900 La Plata, Argentina Fac Ciencias La Plata Argentina RA-1900 ata, RA-1900 La Plata, Argentina
Titolo Testata:
LIPIDS
fascicolo: 12, volume: 35, anno: 2000,
pagine: 1335 - 1344
SICI:
0024-4201(200012)35:12<1335:DCICIM>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
RAT-LIVER MICROSOMES; STEAROYL-COA DESATURASE; FATTY-ACID DEFICIENCY; ERYTHROCYTE-MEMBRANES; FLUORESCENCE; DYNAMICS; PHOSPHOLIPIDS; SEPARATION; RELAXATION; ROTATIONS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Brenner, RR Fac Ciencias, UNLP, CONICET, Inst Invest Bioquim La Plata, Calles 60&120, RA-1900 La Plata, Argentina Fac Ciencias Calles 60&120 La Plata Argentina RA-1900 gentina
Citazione:
A.M. Bernasconi et al., "Dietary cholesterol induces changes in molecular species of hepatic microsomal phosphatidylcholine", LIPIDS, 35(12), 2000, pp. 1335-1344

Abstract

After 21 days on a diet containing 1 g% cholesterol and 0.5 g% cholic acid, rats had an increased content of cholesterol in liver microsomal lipids. In liver, both cholesterol content and Delta9 desaturase activity increased, whereas Delta6 and Delta5 desaturase activities decreased. These changes correlated with increases in oleic, palmitoleic, and linoleic acids and decreases in arachidonic and docosahexenoic acids in total microsomal lipids. Similar fatty acid changes were found in phosphatidylcholine (PC), the principal lipid of the microsomal membrane. In PC the predominant molecular fatty acid species (67% of the total) in the control rats were 18:0/20:4, 16:0/20:4, and 16:0/18:2; and they mainly determined the contribution of PC to the biophysical and biochemical properties of the phospholipid bilayer. Thecholesterol diet decreased specifically the 18:0/20:4 species, and to a lesser extent, 16:0/20:4 and 18:0/22:6. The 18:1-containing species, especially 18:1/18:2 and less so 16:0/18:1 and 18:1/20:4, were increased. A new 18:1/18:1 species appeared. The independent effects of the presence of cholesterol and change of the fatty acid composition of the phospholipid bilayer of liver microsomes on the packing were studied by fluorescence methods using 6-lauroyl-2,4-dimethylaminonaphthalene, 1,6-diphenyl-1,3,5-hexatriene and1-(4-trimethylammonium phenyl)-6-phenyl-1,3,5-hexatriene, which test different parameters and depths of the bilayer. Data showed that the increase ofcholesterol in the membrane, and not the change of the fatty acid composition of phospholipids, was the main determinant of the increased bulk packing of the bilayer. The increase of fluid oleic- and linoleic-containing species almost compensated for the drop in 20:4- and 22:6-containing molecules. But the most important effect was that the general drop in essential n-6 and n-3 polyunsaturated fatty acids meant that this endogenous source for the needs of the animal decreased.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 21:48:24