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Titolo:
Aberrant expression and localization of the cytoskeleton-binding pp52 (LSP1) protein in hairy cell leukemia
Autore:
Miyoshi, EK; Stewart, PL; Kincade, PW; Lee, MB; Thompson, AA; Wall, R;
Indirizzi:
Univ Calif Los Angeles, Sch Med, Dept Microbiol & Immunol, Los Angeles, CA90095 USA Univ Calif Los Angeles Los Angeles CA USA 90095 Los Angeles, CA90095 USA Univ Calif Los Angeles, Sch Med, Dept Mol & Med Pharmacol, Los Angeles, CA90095 USA Univ Calif Los Angeles Los Angeles CA USA 90095 Los Angeles, CA90095 USA Univ Calif Los Angeles, Crump Inst Biol Imaging, Los Angeles, CA 90095 USAUniv Calif Los Angeles Los Angeles CA USA 90095 Los Angeles, CA 90095 USA Oklahoma Med Res Fdn, Oklahoma City, OK 73104 USA Oklahoma Med Res Fdn Oklahoma City OK USA 73104 lahoma City, OK 73104 USA Univ Calif Los Angeles, Inst Mol Biol, Los Angeles, CA 90095 USA Univ Calif Los Angeles Los Angeles CA USA 90095 Los Angeles, CA 90095 USA Univ Calif Los Angeles, Sch Med, Dept Pediat, Div Hematol Oncol, Los Angeles, CA 90095 USA Univ Calif Los Angeles Los Angeles CA USA 90095 Los Angeles, CA 90095 USA Univ Calif Los Angeles, Sch Med, Gwynne Hazen Cherry Mem Labs, Los Angeles, CA 90095 USA Univ Calif Los Angeles Los Angeles CA USA 90095 Los Angeles, CA 90095 USA
Titolo Testata:
LEUKEMIA RESEARCH
fascicolo: 1, volume: 25, anno: 2001,
pagine: 57 - 67
SICI:
0145-2126(200101)25:1<57:AEALOT>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
LYMPHOCYTE-SPECIFIC PROTEIN-1; KINASE-C SUBSTRATE; ACTIN POLYMERIZATION; PLASMA-MEMBRANE; MARCKS FAMILY; CA-2+-BINDING PROTEIN; HUMAN-LEUKOCYTES; SHAPE CHANGES; P50 LSP1; T-CELLS;
Keywords:
pp52; LSP1; phosphoproteins; leukocytes; cell size; microfilament protein; leukemia; hairy cell;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
51
Recensione:
Indirizzi per estratti:
Indirizzo: Wall, R Univ Calif Los Angeles, Sch Med, Dept Microbiol & Immunol, Los Angeles, CA90095 USA Univ Calif Los Angeles Los Angeles CA USA 90095 eles, CA90095 USA
Citazione:
E.K. Miyoshi et al., "Aberrant expression and localization of the cytoskeleton-binding pp52 (LSP1) protein in hairy cell leukemia", LEUK RES, 25(1), 2001, pp. 57-67

Abstract

Non-retractable cell surface projections and cytoskeleton-mediated functional defects are distinguishing features of both hairy cell leukemia (HCL) and neutrophil actin dysfunction (NAD). These defects in NAD neutrophils areattributed to moderate over-expression of pp52 (LSP1), the F-actin-binding, leukocyte-specific phosphoprotein. Here we report that pp52 is similarly elevated in HCL patient PBMCs. Established HCL cell lines exhibited characteristic morphological features like those of fresh HCL cells and showed elevated pp52 levels. The excess pp52 in these HCL cell lines was selectively associated with the F-actin-rich cytoskeletal arrays in surface projections. Treatments producing radical changes in HCL cell shape also altered pp52 expression and intracellular distribution. Alpha interferon (IFN alpha, used to treat HCL) reduced pp52 levels, normalized intracellular pp52 distribution and reverted HCL cells to rounded B cell morphology. Phorbol ester stimulation rapidly generated hyper-phosphorylated pp52 isoforms which translocated from the cytoskeleton to the cytosol prior to the further elongation of surface spikes. This indicates a direct role for phosphorylation in controlling pp52 interactions with the cytoskeleton. Overall, these findings strongly suggest that elevated pp52 expression and/or selective cytoskeletal association contributes to the distinctive morphology of HCL cells. (C) 2001 Elsevier Science Ltd. All rights reserved.

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Documento generato il 29/03/20 alle ore 12:17:03