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Titolo:
Extending unbiased stereology of brain ultrastructure to three-dimensionalvolumes
Autore:
Fiala, JC; Harris, KM;
Indirizzi:
Boston Univ, Dept Biol, Boston, MA 02215 USA Boston Univ Boston MA USA 02215 ton Univ, Dept Biol, Boston, MA 02215 USA
Titolo Testata:
JOURNAL OF THE AMERICAN MEDICAL INFORMATICS ASSOCIATION
fascicolo: 1, volume: 8, anno: 2001,
pagine: 1 - 16
SICI:
1067-5027(200101/02)8:1<1:EUSOBU>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
LONG-TERM POTENTIATION; DENDRITIC SPINES; SERIAL SECTIONS; 3-DIMENSIONAL RECONSTRUCTION; RAT HIPPOCAMPUS; ARBITRARY PARTICLES; ELECTRON-MICROSCOPY; DETERMINING NUMBERS; PYRAMIDAL CELLS; AREA CA1;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Social & Behavioral Sciences
Clinical Medicine
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Fiala, JC Boston Univ, Dept Biol, 5 Cummington St, Boston, MA 02215 USA Boston Univ 5 Cummington St Boston MA USA 02215 on, MA 02215 USA
Citazione:
J.C. Fiala e K.M. Harris, "Extending unbiased stereology of brain ultrastructure to three-dimensionalvolumes", J AM MED IN, 8(1), 2001, pp. 1-16

Abstract

Objective: Analysis of brain ultrastructure is needed to reveal how neurons communicate with one another via synapses and how disease processes alterthis communication. In the past, such analyses have usually been based on single or paired sections obtained by electron microscopy. Reconstruction from multiple serial sections provides a much needed, richer representation of the three-dimensional organization of the brain. This paper introduces anew reconstruction system and new methods for analyzing in three dimensions the location and ultrastructure of neuronal components, such as synapses,which are distributed non-randomly throughout the brain. Design and Measurements: Volumes are reconstructed by defining transformations that align the entire area of adjacent sections. Whole-field alignmentrequires rotation, translation, skew, scaling, and second-order nonlinear deformations. Such transformations are implemented by a linear combination of bivariate polynomials. Computer software for generating transformations based on user input is described. Stereological techniques for assessing structural distributions in reconstructed volumes are the unbiased bricking, disector, unbiased ratio, and per-length counting techniques. A new generalmethod, the fractional counter, is also described. This unbiased techniquerelies on the counting of fractions of objects contained in a test volume. A volume of brain tissue from stratum radiatum of hippocampal area CA1 is reconstructed and analyzed for synaptic density to demonstrate and compare the techniques. Results and Conclusion: Reconstruction makes practicable volume-oriented analysis of ultrastructure using such techniques as the unbiased bricking and fractional counter methods. These analysis methods are less sensitive to the section-to-section variations in counts and section thickness, factors that contribute to the inaccuracy of other stereological methods. In addition, volume reconstruction facilitates visualization and modeling of structures and analysis of three-dimensional relationships such as synaptic connectivity.

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Documento generato il 22/01/20 alle ore 06:58:15