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Titolo:
Two types of protein hydration measured by dielectric dispersion in the gigahertz region, and effects of anesthetics
Autore:
Yoshida, T; Inagaki, H; Kamya, H; Ueda, I;
Indirizzi:
Nagoya Inst Technol, Dept Appl Chem, Showa Ku, Nagoya, Aichi 4668555, Japan Nagoya Inst Technol Nagoya Aichi Japan 4668555 goya, Aichi 4668555, Japan DVA Med Ctr, Anesthesia 112A, Salt Lake City, UT 84148 USA DVA Med Ctr Salt Lake City UT USA 84148 12A, Salt Lake City, UT 84148 USA
Titolo Testata:
JOURNAL OF PHYSICAL CHEMISTRY B
fascicolo: 1, volume: 105, anno: 2001,
pagine: 256 - 260
SICI:
1520-6106(20010111)105:1<256:TTOPHM>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLARIZATION; ALCOHOLS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Yoshida, T Nagoya Inst Technol, Dept Appl Chem, Showa Ku, Nagoya, Aichi 4668555, Japan Nagoya Inst Technol Nagoya Aichi Japan 4668555 4668555, Japan
Citazione:
T. Yoshida et al., "Two types of protein hydration measured by dielectric dispersion in the gigahertz region, and effects of anesthetics", J PHYS CH B, 105(1), 2001, pp. 256-260

Abstract

Two types of hydration of bovine serum albumin, BSA, were found by microwave impedance dispersion according to Suzuhi et al. (J. Phys Chen r. 1996, 100, 7279). At 25.0 degreesC the number of the strongly bound water molecules, N-s, was 606 and that of the weakly bound water molecules, N-w, was 393 per one BSA molecule. Elevation of the temperature decreased N-s continuously, whereas it increased N-w discontinuously at 30 degreesC. Addition of ananesthetic halothane increased N, at the expense of N-w. At halothane 2.5 mM, N-s decreased by 3001 and N-W increased by about 350. The electrical conductance of BSA solution showed a minimum at about 1.0 mM halothane. The halothane concentration that showed the minimum electrical conductance coincided with the maximum in N-W. Because the volume fraction of BSA is less than 2.5%, the conductance change is attributable to the change of bulk ion concentrations. The initial decrease of conductance represents the suppression of ionization by halothane. The increase of conductance above 1.0 mM halothane indicates unfolding of the protein, exposing polar moieties to water, thus increasing the ion concentration.

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Documento generato il 13/07/20 alle ore 07:59:43