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Titolo:
NMR spectroscopic evidence for Mn2+(Mg2+) binding to a precursor-tRNA microhelix near the potential RNase P cleavage site
Autore:
Zuleeg, T; Hartmann, RK; Kreutzer, R; Limmer, S;
Indirizzi:
Univ Bayreuth, Biochem Lab, D-95440 Bayreuth, Germany Univ Bayreuth Bayreuth Germany D-95440 em Lab, D-95440 Bayreuth, Germany Med Univ Lubeck, Inst Biochem, D-23538 Lubeck, Germany Med Univ Lubeck Lubeck Germany D-23538 Biochem, D-23538 Lubeck, Germany Ribopharma AG, D-95447 Bayreuth, Germany Ribopharma AG Bayreuth Germany D-95447 rma AG, D-95447 Bayreuth, Germany
Titolo Testata:
JOURNAL OF MOLECULAR BIOLOGY
fascicolo: 2, volume: 305, anno: 2001,
pagine: 181 - 189
SICI:
0022-2836(20010112)305:2<181:NSEFMB>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
METAL-ION BINDING; ESCHERICHIA-COLI; HAMMERHEAD RIBOZYME; RIBONUCLEASE-P; MAGNESIUM-IONS; ACCEPTOR STEM; THERMODYNAMICS; CONFORMATION; SUBUNIT; PAIR;
Keywords:
RNase P; cleavage; precursor-tRNA; divalent metal ion; NMR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Limmer, S Univ Bayreuth, Biochem Lab, Univ Str 30, D-95440 Bayreuth, Germany Univ Bayreuth Univ Str 30 Bayreuth Germany D-95440 uth, Germany
Citazione:
T. Zuleeg et al., "NMR spectroscopic evidence for Mn2+(Mg2+) binding to a precursor-tRNA microhelix near the potential RNase P cleavage site", J MOL BIOL, 305(2), 2001, pp. 181-189

Abstract

The binding of Mg2+/Mn2+ to acceptor stem microhelices as minimal models for precursor-tRNA(Gly) is demonstrated by NMR spectroscopy. From the evaluation of COSY and NOESY spectra, binding sites for Mg2+/Mn2+ can be inferred. In particular, one binding site exists near the ribose moiety of nucleotide-1 at the position of cleavage by RNase P. From comparison with a variantpossessing a deoxynucleotide at this position, it is concluded that the 2'-OH group of this nucleotide is indispensable for coordinating the divalentmetal ion. Hence, this catalytically important metal ion is "pre-bound" tothe precursor-tRNA before complexation with RNase P. (C) 2001 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 16/07/20 alle ore 19:05:44