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Titolo:
Use of an allele-specific polymerase chain reaction assay to genotype pyrethroid resistant strains of Boophilus microplus (Acari : Ixodidae)
Autore:
Guerrero, FD; Davey, RB; Miller, RJ;
Indirizzi:
ARS, Knipling Bushland US Livestock Insects Res Lab, USDA, Kerrville, TX 78028 USA ARS Kerrville TX USA 78028 Insects Res Lab, USDA, Kerrville, TX 78028 USA
Titolo Testata:
JOURNAL OF MEDICAL ENTOMOLOGY
fascicolo: 1, volume: 38, anno: 2001,
pagine: 44 - 50
SICI:
0022-2585(200101)38:1<44:UOAAPC>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
SODIUM-CHANNEL GENE; CATTLE TICK; HAEMATOBIA-IRRITANS; POINT MUTATIONS; KDR; IDENTIFICATION; INSECTICIDES; POPULATIONS; LEPIDOPTERA;
Keywords:
polymerase chain reaction; resistance mechanism; diagnostic; sodium channel; mutation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Guerrero, FD ARS, Knipling Bushland US Livestock Insects Res Lab, USDA, Kerrville, TX 78028 USA ARS Kerrville TX USA 78028 ab, USDA, Kerrville, TX 78028 USA
Citazione:
F.D. Guerrero et al., "Use of an allele-specific polymerase chain reaction assay to genotype pyrethroid resistant strains of Boophilus microplus (Acari : Ixodidae)", J MED ENT, 38(1), 2001, pp. 44-50

Abstract

A polymerase chain reaction-based assay was developed to detect the presence of a pyrethroid resistance-associated amino acid substitution in Boophilus microplus (Canestrini). The assay uses a simple method for the extraction of genomic DNA from individual larvae and genotypes individuals for the presence of a Phe --> Ile amino acid substitution in the S6 transmembrane segment of domain III of the para-like sodium channel, clearly distinguishingheterozygotes from homozygotes. High frequencies for this amino acid substitution were found in the Corrales and San Felipe strains, which have target site insensitivity mechanisms for pyrethroid resistance. The Caporal resistant strain contained lower yet substantial numbers of amino acid-substituted alleles. Low amino acid substitution frequencies were found in the susceptible reference Gonzales strain and the Coatzacoalcos strain, which has metabolic esterase-mediated pyrethroid resistance. The amino acid substitution was not found in six other strains that were susceptible to pyrethroids.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/04/20 alle ore 19:53:52