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Titolo:
Green fluorescent protein-tagged Leishmania in phlebotomine sand flies
Autore:
Guevara, P; Pinto-Santini, D; Rojas, A; Crisante, G; Anez, N; Ramirez, JL;
Indirizzi:
Cent Univ Venezuela, Fac Ciencias, Inst Expt Biol, Caracas 1041A, Venezuela Cent Univ Venezuela Caracas Venezuela 1041A ol, Caracas 1041A, Venezuela Univ Los Andes, Fac Ciencias, Lab Invest Parasitol Jose Francisco Torrealba, Merida 5101, Venezuela Univ Los Andes Merida Venezuela 5101 o Torrealba, Merida 5101, Venezuela
Titolo Testata:
JOURNAL OF MEDICAL ENTOMOLOGY
fascicolo: 1, volume: 38, anno: 2001,
pagine: 39 - 43
SICI:
0022-2585(200101)38:1<39:GFPLIP>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANSFECTION; EXPRESSION; SANDFLIES; PROMOTER; GENES;
Keywords:
sand fly; green fluorescent-Leishmania; nonaxenic flies;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
13
Recensione:
Indirizzi per estratti:
Indirizzo: Ramirez, JL Cent Univ Venezuela, Fac Ciencias, Inst Expt Biol, Caracas 1041A, Venezuela Cent Univ Venezuela Caracas Venezuela 1041A 1041A, Venezuela
Citazione:
P. Guevara et al., "Green fluorescent protein-tagged Leishmania in phlebotomine sand flies", J MED ENT, 38(1), 2001, pp. 39-43

Abstract

In this work we have used for the first time green fluorescent protein (GFP) tagged cells of the human parasite Leishmania donovani to observe its development in the gut of phlebotomine sand flies. Low numbers of GFP-tagged L. donovani were more easily detected than nontagged Leishmania, suggestingthat GFP-tagged Leishmania could be used to efficiently study the biology of Leishmania in their vectors, and open the possibility of using nonaxenicflies. Using this method, we found that GFP-tagged L, donovani, the ethiological agent of Old World Kala-azar, were able to establish an infection within the gut of Lutzomyia species, which are vectors of New World Leishmania. The GFP-tagged parasites divide successfully in the gut of colonized andin wild caught Lu. longipalpis (Lutz & Neiva, 1912), Lu. ovallesis (Ortiz,1952), and Lu. youngi (Feliciangeli & Murillo, 1985). In the case of Lulongipalpis the labeled parasite exhibited a normal anterior development as the one observed in its natural vector.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/10/20 alle ore 08:50:49